轴突导向因子Slit及其受体Robo在损伤周围神经的表达差异及其功能
发布时间：2018-09-12 19:01
【摘要】：Slit为中枢神经系统中线胶质细胞所分泌的一类可扩散的大分子蛋白质，具有排斥发育中的神经生长锥，诱导细胞迁移，促进轴突生长与分支形成的作用。Robo属于细胞粘附分子(cell adhesionmolecules，CAMs)免疫球蛋白(immunoglobulin，Ig)超家族成员，为跨膜受体蛋白，介导Slit对生长锥和神经元的排斥作用，参与调控发育中的中枢神经轴突定向，同时Robo也具有同亲和附着与异亲和附着特性，可促进轴突生长和调控神经纤维束化、定位。有关Slit及Robo在成年动物周围神经系统中的分布与功能报道极少。同时，周围神经再生特异性(再生轴突生长定向)的细胞与分子机制尚不明确。 目的：研究Slit、Robo基因和蛋白质在成年大鼠脊髓、脊神经节(dorsal root ganglian，DRG)、坐骨神经(sciatic nerve，SN)的表达差异及损伤后的变化，探讨它们在神经损伤再生过程中的作用，揭示Slit、Robo的生理功能和周围神经再生特异的分子机制。 材料与方法：SD大鼠坐骨神经切断或脊神经后根切断后，存活3、7、14、21、28 d，，取其相应节段脊髓、后根、DRG和损伤神经的远、近端；利用RT-PCR、原位杂交、免疫组织化学等方法检测Slit1、Slit2、Robo1、Robo2在上述组织中的表达。进而利用坐骨神经压榨(sciatic nerve crush，SNC)和切断后立即缝合(end to end suturetransected sciatic nerve，SNS)模型，将可溶性Robo1-Fc融合蛋白注入到损伤或缝合部位，形成坐骨神经压榨实验组(SNCE)和坐骨神
[Abstract]:Slit is a kind of diffusible macromolecular protein secreted by glial cells in the central nervous system. Promoting axon growth and branching. Robo belongs to the superfamily of cell adhesion molecule (cell adhesionmolecules,CAMs) immunoglobulin (immunoglobulin,Ig), which is a transmembrane receptor protein that mediates the rejection of growth cone and neurons by Slit. It is involved in the regulation of the orientation of central nervous axons during development, and Robo also has the characteristics of homocompatibility and hetero-affinity, which can promote the growth of axons and regulate the bundles of nerve fibers. The distribution and function of Slit and Robo in the peripheral nervous system of adult animals are rarely reported. At the same time, the cellular and molecular mechanisms of peripheral nerve regeneration specificity (regenerative axon growth orientation) are unclear. Objective: to study the expression of Slit,Robo gene and protein in spinal cord, spinal ganglia (dorsal root ganglian,DRG) and sciatic nerve (sciatic nerve,SN) of adult rats and their role in the regeneration of nerve injury. To reveal the physiological function of Slit,Robo and the specific molecular mechanism of peripheral nerve regeneration. Materials and methods after sciatic nerve transection or spinal nerve posterior root transection, the rats survived for 28 days, and the corresponding segments of spinal cord, posterior root and the distal and proximal end of injured nerve were taken from the corresponding segments of spinal cord, posterior root and injured nerve, and RT-PCR, in situ hybridization was used. Immunohistochemical methods were used to detect the expression of Slit1,Slit2,Robo1,Robo2 in these tissues. Then the model of sciatic nerve compression (sciatic nerve crush,SNC) and (end to end suturetransected sciatic nerve,SNS (suture immediately after transection) was used to inject soluble Robo1-Fc fusion protein into the injured or sutured site to form the sciatic nerve compression experimental group (SNCE) and ischium spirit.
【学位授予单位】：中南大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R322
本文编号：2239942
[Abstract]:Slit is a kind of diffusible macromolecular protein secreted by glial cells in the central nervous system. Promoting axon growth and branching. Robo belongs to the superfamily of cell adhesion molecule (cell adhesionmolecules,CAMs) immunoglobulin (immunoglobulin,Ig), which is a transmembrane receptor protein that mediates the rejection of growth cone and neurons by Slit. It is involved in the regulation of the orientation of central nervous axons during development, and Robo also has the characteristics of homocompatibility and hetero-affinity, which can promote the growth of axons and regulate the bundles of nerve fibers. The distribution and function of Slit and Robo in the peripheral nervous system of adult animals are rarely reported. At the same time, the cellular and molecular mechanisms of peripheral nerve regeneration specificity (regenerative axon growth orientation) are unclear. Objective: to study the expression of Slit,Robo gene and protein in spinal cord, spinal ganglia (dorsal root ganglian,DRG) and sciatic nerve (sciatic nerve,SN) of adult rats and their role in the regeneration of nerve injury. To reveal the physiological function of Slit,Robo and the specific molecular mechanism of peripheral nerve regeneration. Materials and methods after sciatic nerve transection or spinal nerve posterior root transection, the rats survived for 28 days, and the corresponding segments of spinal cord, posterior root and the distal and proximal end of injured nerve were taken from the corresponding segments of spinal cord, posterior root and injured nerve, and RT-PCR, in situ hybridization was used. Immunohistochemical methods were used to detect the expression of Slit1,Slit2,Robo1,Robo2 in these tissues. Then the model of sciatic nerve compression (sciatic nerve crush,SNC) and (end to end suturetransected sciatic nerve,SNS (suture immediately after transection) was used to inject soluble Robo1-Fc fusion protein into the injured or sutured site to form the sciatic nerve compression experimental group (SNCE) and ischium spirit.
【学位授予单位】：中南大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R322
【引证文献】
相关期刊论文 前1条
1 吕凯;李凤;龚标;代恩泽;汪莹;曾志华;;电针对局灶性脑梗死大鼠皮质Slit2/Robo1表达的影响[J];针刺研究;2013年04期
相关硕士学位论文 前2条
1 赵欢;脑泰通颗粒对缺血性脑卒中模型大鼠Slit2及VEGF影响的实验研究[D];陕西中医学院;2010年
2 李霖;急性肺损伤大鼠肺神经导向因子Slit2及Robo4表达的研究[D];南华大学;2013年
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