树突状细胞诱导半相合骨髓移植免疫耐受的实验研究

发布时间：2018-09-14 08:02

【摘要】： 目的探讨利用未成熟树突状细胞诱导半相合骨髓移植免疫耐受的疗效,寻找防治骨髓移植免疫排斥反应的新方法。方法①以雄性SD大鼠为骨髓移植供鼠,以雄性SD大鼠与雌性Wistar大鼠的杂交雌鼠(F1雌鼠)为骨髓移植受鼠建立半相合骨髓移植动物模型。②用供鼠的外周血在体外经细胞因子rmGM-CSF、rmIL-4诱导培养7天收获未成熟树突状细胞。③实验组受鼠10只,接受骨髓移植+供鼠来源的未成熟树突状细胞输注;对照组受鼠10只,只接受骨髓移植,无未成熟树突状细胞的输注;观察比较aGVHD的发病情况。结果①观察至移植后+40天,存活的16只受鼠中有15只表达SRY基因。②用供鼠外周血细胞诱导培养imDC的过程中,经动态跟踪观察显示:培养细胞的形态学特点符合未成熟树突状细胞的形态学特点。③实验组受鼠的aGVHD发生率显著低于对照组(60%VS100%)。④观察至移植后+40天,实验组受鼠的临床aGVHD评分显著低于对照组(2.7±1.0 VS 6.9±1.5);实验组受鼠的肝脏、小肠病理GVHD严重程度显著低于对照组。结论①利用SD雄鼠和F1雌鼠(雄性SD大鼠与雌性Wrist大鼠的杂交雌鼠)建立半相合骨髓移植动物模型进行有关研究是可行的。②利用大鼠外周血诱导培养未成熟树突状细胞可用于其诱导移植免疫耐受性的研究。③未成熟树突状细胞可降低大鼠半相合骨髓移植后aGVHD的发生率、减轻大鼠半相合骨髓移植后aGVHD的病情。
[Abstract]:Objective to investigate the effect of immature dendritic cells on the induction of haploidentical bone marrow transplantation (BMT) immune tolerance and to find a new method to prevent and treat the immune rejection of bone marrow transplantation (BMT). Methods 1 male SD rats were used as bone marrow donors. Establishment of haploidentical bone marrow transplantation animal model using male SD rat and female Wistar rat hybrid female rat (F1 female rat) as bone marrow transplant recipients. 2. The peripheral blood of donor rat was cultured in vitro with cytokine rmGM-CSF,rmIL-4 for 7 days to harvest immature Dendritic cells (DSCs) of the experimental group (n = 10), The immature dendritic cells from donor mice were injected with bone marrow transplantation, while the control group (n = 10) received bone marrow transplantation only, and there was no infusion of immature dendritic cells. The incidence of aGVHD was observed and compared. Results (1) after 40 days of transplantation, 15 of the 16 surviving recipient mice expressed SRY gene and imDC was induced by donor peripheral blood cells. The morphological characteristics of cultured cells were consistent with the morphological characteristics of immature dendritic cells. 3 the incidence of aGVHD in the experimental group was significantly lower than that in the control group (60 vs 100%) until 40 days after transplantation. The clinical aGVHD score of the experimental group was significantly lower than that of the control group (2.7 卤1.0 VS, 6.9 卤1.5), and the severity of pathological GVHD in the liver and small intestine of the experimental group was significantly lower than that of the control group. Conclusion 1 it is feasible to establish haploidentical bone marrow transplantation animal model by using SD male and F1 female (male SD rat and female Wrist rat hybrid female rat). 2 it is feasible to use peripheral blood of rat to induce immature tree. Induction of immune tolerance by dendritic cells. 3 immature dendritic cells can reduce the incidence of aGVHD after haploidentical bone marrow transplantation in rats. To alleviate the condition of aGVHD after haploidentical bone marrow transplantation in rats.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

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