受损视神经大胶质细胞的去分化及预变性周围神经的诱导

发布时间：2018-09-18 21:09

【摘要】：本文通过建立大鼠视神经损伤模型,应用免疫组织化学、原位杂交组织化学、免疫荧光双标组织化学和基因芯片的方法从形态学、蛋白表达和基因表达的角度研究视神经损伤和/或移植自体预变性腓总神经后神经胶质细胞和神经再生的变化,旨在探讨受损视神经大胶质细胞去分化及诱导的可行性。结果发现视神经伤后巢蛋白表达升高,胶质细胞基因表达发生变化等;移植周围神经后巢蛋白表达进一步升高,并能影响胶质细胞基因表达等。观察到神经胶质细胞中巢蛋白分别与GFAP、MBP共存。结果提示视神经吸断后,其微环境不利于神经再生,胶质细胞基因表达发生变化,大胶质细胞呈去分化趋势;移植周围神经改善了视神经损伤后抑制性微环境,影响胶质细胞基因表达,并诱导大胶质细胞去分化。
[Abstract]:In this paper, a rat model of optic nerve injury was established. The methods of immunohistochemistry, in situ hybridization histochemistry, immunofluorescence double labeling histochemistry and gene chip were used from morphology. The changes of glial cells and nerve regeneration after optic nerve injury and / or autograft predenaturation of common peroneal nerve were studied from the angle of protein expression and gene expression in order to explore the feasibility of dedifferentiation and induction of injured optic nerve macroglial cells. The results showed that the expression of nestin increased and the gene expression of glial cells changed after optic nerve injury, and the expression of nestin increased further after transplantation of peripheral nerve and affected the gene expression of glial cells. It was observed that nestin coexisted with GFAP,MBP in glial cells. The results suggested that the microenvironment of optic nerve was not conducive to nerve regeneration, the gene expression of glial cells was changed, the tendency of macroglial cells was dedifferentiated, and the transplanted peripheral nerve improved the inhibitory microenvironment after optic nerve injury. It affects gene expression of glial cells and induces dedifferentiation of macroglia cells.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R361

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