长期高脂饲养对SD大鼠主动脉内皮依赖性血管舒张功能的影响及机制研究
发布时间：2018-10-12 08:48
【摘要】： 目的：观察28周高脂饮食对SD大鼠外周组织胰岛素敏感性及血管内皮舒张功能的影响。 方法：41只SD雄性大鼠随机分为高脂饲料喂养组(HF，n=21)和普通饲料喂养组(NC，n=20)，喂养28周后，做以下检查：(1)体重及腹内脂肪称重：(2)采血检测空腹血糖(FBG)、胰岛素(FINS)、游离脂肪酸(FFA)、甘油三酯(TG)等：(3)正常血糖高胰岛素钳夹试验评价外周胰岛素抵抗程度和(4)取出主动脉置于器官浴槽中，平衡后先经PGF2α预收缩，再分别加入乙酰胆碱及硝普钠，观察主动脉环对其舒张反应。 结果：(1) 28周时HF组体重及腹内脂肪含量升高：(2)与NC组相比，HF组血FBG和FINS水平增高，血FFA和TG也明显增高(p＜0.05)；(3) HF组葡萄糖输注率(GIR)为3.60±1.2 mg·min~(-1)·kg~(-1)，，明显低于NC组11.94±1.7mg·min~(-1)·kg~(-1)(p＜0.01)；(4)与NC组相比，HF组内皮依赖性血管舒张(EDV)明显降低(81.3±3.1％vs 35.2±2.4％，p＜0.01)，而非内皮依赖性血管舒张(NEDV)两组无明显差别(85.4±3.2％vs 72.3±2.2％，p＞0.05)和(5)相关分析显示：HF组腹内脂肪重量及血FFA水平与GIR呈负相关，HF组血FFA水平与内皮依赖性血管舒张(EDV)呈负相关。 结论：长期高脂喂养可导致大鼠外周组织胰岛素抵抗和内皮依赖性血管舒张功能受损，其机理与长期高脂喂养导致大鼠血FFA水平升高有关。 目的：观察28周高脂饲养对SD大鼠主动脉内皮依赖性血管舒张功能影响的机制。 方法：8周龄雄性SD大鼠41只随机分为2组：正常饲料喂养组(NC，n=20)和高脂饲料喂养组(HF，n=21)，喂养28周后，做以下检查：(1)采血检测MDA、GSH水平；(2)检测C-反应蛋白(CRP)水平；(3)检测血管细胞间粘附因子-1(sVCAM-1)水平：(4)评价离体主动脉环对乙酰胆碱及硝普钠的舒张反应和(5)采用荧光定量PCR检测主动脉内皮细胞eNOSmRNA表达。 结果：(1)与NC组相比，HF组血MDA水平明显增高(16.86±4.5 vs26.63±5.0 nmol／ml，p＜0.01)，血GSH水平降低(124.10±34.7 vs84.78±21.0mg／ml，p＜0.01)；(2) HF组血CRP水平高于NC组(0.71±0.23mg／L vs 0.53±0.14 mg／L，p＜0.05)；(3)与NC组相比，HF组血sVCAM-1水平明显增高(344.1±84.5 vs 465.3±124.6ng／mlnmol／ml，p＜0.01)；(4)与NC组相比，HF组内皮依赖性血管舒张(EDV)明显降低(81.3±3.1％vs 35.2±2.4％，p＜0.01)，而非内皮依赖性血管舒张(NEDV)两组无明显差别(85.4±3.2％vs 72.3±2.2％，p＞0.05)和(5)与NC组相比，HF组主动脉eNOSmRNA表达降低了34％。 结论：长期高脂饲养SD大鼠存在内皮依赖性血管舒张功能减低，可能是高FFA血症抑制血管内皮细胞eNOS表达而引起内皮细胞NO产生和释放减少所致，其机制与FFA所诱导的氧化应激和炎症反应有关。
[Abstract]:Aim: to observe the effects of 28 weeks high fat diet on insulin sensitivity and vascular endothelial diastolic function in peripheral tissues of SD rats. Methods: 41 male SD rats were randomly divided into high fat diet (HF,n=21) group and general feed feeding (NC,n=20) group. Do the following tests: (1) body weight and abdominal fat weighing; (2) fasting blood glucose, (FBG), insulin, (FINS), free fatty acid, (FFA), triglyceride (TG) et al; (3) normal hyperglycemic hyperinsulinemic clamp test to evaluate the degree of peripheral insulin resistance; (4) Take out the aorta and put it in an organ bath. After equilibrium, the aortic rings were precontracted by PGF2 伪, then acetylcholine and sodium nitroprusside were added respectively to observe the diastolic response of aortic rings. Results: (1) after 28 weeks, the body weight and abdominal fat content in HF group increased: (2) compared with NC group, the serum FBG and FINS levels in HF group were higher than those in NC group (p < 0. 05); (3). The (GIR) infusion rate in HF group was 3.60 卤1.2 mg min~ (-1) kg~ (-1), which was significantly lower than that in NC group (11.94 卤1.7mg min~ (-1) kg~ (-1) (p < 0.01). (4) compared with NC group, (EDV) of endothelium-dependent vasodilation in HF group was significantly lower than that in NC group (81.3 卤3.1%vs 35.2 卤2.4, p < 0.01), but there was no significant difference between HF group and non-endothelium-dependent vasodilation (NEDV) group (85.4 卤3.2%vs 72.3 卤2.2, P > 0.05). Fat weight and serum FFA level were negatively correlated with GIR, and FFA levels were negatively correlated with endothelium-dependent vasodilation (EDV) in HF group. Conclusion: Long-term high fat feeding can lead to insulin resistance in peripheral tissues and impaired endothelium-dependent vasodilation function in rats. The mechanism is related to the increase of FFA level in blood of rats induced by long-term high fat feeding. Aim: to observe the mechanism of 28 weeks high fat feeding on endothelium-dependent vasodilation of aorta in SD rats. Methods: Forty-one 8-week-old male SD rats were randomly divided into two groups: normal feed feeding group (NC,n=20) and high-fat feed feeding group (HF,n=21). After 28 weeks of feeding, the following examinations were performed: (1) the level of MDA,GSH was detected by blood collection, (2) the level of C-reactive protein (CRP) was detected. (3) the levels of vascular intercellular adhesion factor-1 (sVCAM-1) were measured: (4) the relaxation response of isolated aortic rings to acetylcholine and sodium nitroprusside was evaluated; (5) the expression of eNOSmRNA in aortic endothelial cells was detected by fluorescence quantitative PCR. Results: (1) compared with the NC group, the serum MDA level in the HF group was significantly higher (16.86 卤4.5 vs26.63 卤5.0 nmol/ml,p < 0. 01) and the serum GSH level was decreased (124.10 卤34.7 vs84.78 卤21.0 mg / ml); (2). The CRP level in the HF group was higher than that in the NC group (0. 71 卤0.23mg/L vs 0. 53 卤0. 14 mg/L,p < 0. 05). (3) compared with NC group, the serum sVCAM-1 level in HF group was significantly higher than that in NC group (344.1 卤84.5 vs, 465.3 卤124.6 ngmol / ml, P < 0. 01). (4) compared with NC group, (EDV) of endothelium-dependent vasodilation in HF group was significantly lower than that in NC group (81.3 卤3.1%vs 35.2 卤2.4, p < 0.01), but there was no significant difference between (NEDV) group and non-endothelium-dependent vasodilation (NEDV) group (85.4 卤3.2%vs 72.3 卤2.2, P > 0.05) and (5) compared with NC group, aortic eNOSmRNA in HF group was significantly lower than that in NC group. The expression was reduced by 34%. Conclusion: there is a decrease in endothelium-dependent vasodilation function in SD rats fed with hyperlipidemia for a long time, which may be due to the inhibition of eNOS expression in vascular endothelial cells by hyperlipidemia and the decrease of NO production and release in endothelial cells. Its mechanism is related to oxidative stress and inflammation induced by FFA.
【学位授予单位】：中国协和医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363
本文编号：2265515
[Abstract]:Aim: to observe the effects of 28 weeks high fat diet on insulin sensitivity and vascular endothelial diastolic function in peripheral tissues of SD rats. Methods: 41 male SD rats were randomly divided into high fat diet (HF,n=21) group and general feed feeding (NC,n=20) group. Do the following tests: (1) body weight and abdominal fat weighing; (2) fasting blood glucose, (FBG), insulin, (FINS), free fatty acid, (FFA), triglyceride (TG) et al; (3) normal hyperglycemic hyperinsulinemic clamp test to evaluate the degree of peripheral insulin resistance; (4) Take out the aorta and put it in an organ bath. After equilibrium, the aortic rings were precontracted by PGF2 伪, then acetylcholine and sodium nitroprusside were added respectively to observe the diastolic response of aortic rings. Results: (1) after 28 weeks, the body weight and abdominal fat content in HF group increased: (2) compared with NC group, the serum FBG and FINS levels in HF group were higher than those in NC group (p < 0. 05); (3). The (GIR) infusion rate in HF group was 3.60 卤1.2 mg min~ (-1) kg~ (-1), which was significantly lower than that in NC group (11.94 卤1.7mg min~ (-1) kg~ (-1) (p < 0.01). (4) compared with NC group, (EDV) of endothelium-dependent vasodilation in HF group was significantly lower than that in NC group (81.3 卤3.1%vs 35.2 卤2.4, p < 0.01), but there was no significant difference between HF group and non-endothelium-dependent vasodilation (NEDV) group (85.4 卤3.2%vs 72.3 卤2.2, P > 0.05). Fat weight and serum FFA level were negatively correlated with GIR, and FFA levels were negatively correlated with endothelium-dependent vasodilation (EDV) in HF group. Conclusion: Long-term high fat feeding can lead to insulin resistance in peripheral tissues and impaired endothelium-dependent vasodilation function in rats. The mechanism is related to the increase of FFA level in blood of rats induced by long-term high fat feeding. Aim: to observe the mechanism of 28 weeks high fat feeding on endothelium-dependent vasodilation of aorta in SD rats. Methods: Forty-one 8-week-old male SD rats were randomly divided into two groups: normal feed feeding group (NC,n=20) and high-fat feed feeding group (HF,n=21). After 28 weeks of feeding, the following examinations were performed: (1) the level of MDA,GSH was detected by blood collection, (2) the level of C-reactive protein (CRP) was detected. (3) the levels of vascular intercellular adhesion factor-1 (sVCAM-1) were measured: (4) the relaxation response of isolated aortic rings to acetylcholine and sodium nitroprusside was evaluated; (5) the expression of eNOSmRNA in aortic endothelial cells was detected by fluorescence quantitative PCR. Results: (1) compared with the NC group, the serum MDA level in the HF group was significantly higher (16.86 卤4.5 vs26.63 卤5.0 nmol/ml,p < 0. 01) and the serum GSH level was decreased (124.10 卤34.7 vs84.78 卤21.0 mg / ml); (2). The CRP level in the HF group was higher than that in the NC group (0. 71 卤0.23mg/L vs 0. 53 卤0. 14 mg/L,p < 0. 05). (3) compared with NC group, the serum sVCAM-1 level in HF group was significantly higher than that in NC group (344.1 卤84.5 vs, 465.3 卤124.6 ngmol / ml, P < 0. 01). (4) compared with NC group, (EDV) of endothelium-dependent vasodilation in HF group was significantly lower than that in NC group (81.3 卤3.1%vs 35.2 卤2.4, p < 0.01), but there was no significant difference between (NEDV) group and non-endothelium-dependent vasodilation (NEDV) group (85.4 卤3.2%vs 72.3 卤2.2, P > 0.05) and (5) compared with NC group, aortic eNOSmRNA in HF group was significantly lower than that in NC group. The expression was reduced by 34%. Conclusion: there is a decrease in endothelium-dependent vasodilation function in SD rats fed with hyperlipidemia for a long time, which may be due to the inhibition of eNOS expression in vascular endothelial cells by hyperlipidemia and the decrease of NO production and release in endothelial cells. Its mechanism is related to oxidative stress and inflammation induced by FFA.
【学位授予单位】：中国协和医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363
【参考文献】
相关期刊论文 前8条
1 李宏亮,余叶蓉,喻红玲,王椿,张祥迅;单纯性肥胖患者外周胰岛素抵抗与兀细胞功能的研究[J];四川大学学报(医学版);2005年03期
2 方臻飞,周胜华,胡信群,沈向前,祁述善;急性冠状动脉综合征患者冠状动脉循环粘附分子的变化[J];中国动脉硬化杂志;2005年02期
3 吕琳,鹿克风,罗静,朱兴雷;氟伐他汀对兔动脉粥样硬化血管壁NF-κB及ICAM-1表达的影响[J];山东大学学报(医学版);2005年04期
4 周庆,黄波,杨震;阿托伐他汀对冠心病患者血清细胞因子水平影响的研究[J];现代临床医学生物工程学杂志;2005年04期
5 陶玲,刘铭,宋少莉;高脂血症患者粘附分子水平及调脂干预的研究[J];中国心血管杂志;2004年02期
6 杨立森,石学宁,方永鹏,李玉梅,潘月英,林媛豪,陈树兰;冠心病血清可溶性粘附分子水平与血脂异常的关系[J];宁夏医学院学报;2003年05期
7 秦晋梅,杨波,李建军,陈莉,戴淑华;氟伐他汀对高脂血症兔心肌缺血再灌注损伤心肌局部细胞间粘附分子-1的影响[J];中国临床药理学与治疗学;2004年11期
8 余叶蓉,朱锦舒,吴永刚,Alain D Baron;血游离脂肪酸水平对SD大鼠主动脉舒张功能的影响[J];中华内分泌代谢杂志;2002年01期
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