兔实验性颈动脉瘤模型的建立及MMP-1、MMP-2、MMP-9、TIMP-2在模型中的表达研究

发布时间：2018-10-12 08:46

【摘要】：研究背景和目的:脑动脉瘤(aneurysm,AN)是自发性蛛网膜下腔出血最常见的原因之一,其破裂出血的发病率在脑血管疾病中占第三位,仅次于脑血栓形成和高血压脑出血,其出血后第一个月的死亡率可高达近50%。研究AN的发病机制对于预防AN的形成、控制AN的扩大、破裂、改善AN的预后都会有很大帮助。如何建立理想的AN动物模型,是开展此类研究的难点和关键。目前脑AN制作的方法很多,主要有外科手术法、介入制作法、血流动力学和药物诱导法等。这些方法所制作的AN模型离理想的AN模型尚有一定差距。弹力蛋白酶(elastase,EA)动脉外壁滴注所诱发的AN与人脑AN病理改变基本一致,其方法简单,容易重复,成本低廉。但目前对所使用的EA溶液的浓度、量,结扎一侧颈总动脉对AN形成情况的影响,AN形成早期的形态学变化,AN形成后的长期效果尚不太清楚和有一定争议。探讨合适的经动脉外壁滴注的EA溶液的浓度、量,结扎一侧颈总动脉(common carotid artery,CCA)对AN形成情况的影响,AN形成早期的形态学变化,AN形成后的长期效果,为进一步探讨AN的发病机制和治疗措施打下基础。方法:①新西兰白兔12只,雌雄各半,随机分成4组,测量颈外动脉与CCA分叉处近端0.5mm处及其沿CCA近心方向各相隔1cm的4点的血管直径;双侧分次分别滴注0.5u/μl、1.0u/μl、1.5u/μl、3.0u%q
[Abstract]:Background and objective: cerebral aneurysm (aneurysm,AN) is one of the most common causes of spontaneous subarachnoid hemorrhage. The death rate in the first month after bleeding can be as high as nearly 50. Studying the pathogenesis of AN is of great help to prevent the formation of AN, control the expansion of AN, rupture and improve the prognosis of AN. How to establish the ideal AN animal model is the difficulty and key to carry out this kind of research. At present, there are many methods to make brain AN, such as surgery, interventional method, hemodynamics and drug induction. The AN model made by these methods is still far from the ideal AN model. The pathological changes of AN induced by arterial infusion of elastase (elastase,EA) are basically the same as that of human brain AN. The method is simple, easy to repeat and low in cost. However, the concentration and quantity of EA solution, the effect of ligation of common carotid artery on the formation of AN, the morphological changes in the early stage of AN formation, and the long-term effect after AN formation are still unclear and controversial. To investigate the effect of appropriate concentration and quantity of EA solution dripping through the external wall of artery and ligation of unilateral common carotid artery (common carotid artery,CCA) on the formation of AN, the morphological changes in the early stage of AN formation, and the long-term effect after AN formation. To further explore the pathogenesis of AN and treatment measures lay a foundation. Methods: 1Twelve New Zealand white rabbits, half male and half female, were randomly divided into 4 groups. The diameter of the blood vessels at the proximal end of the 0.5mm at the external carotid artery and the CCA bifurcation and the four points separated by the 1cm in the proximal direction of the CCA were measured, and 0.5u/ 渭 l 1.0 u / 渭 l 1. 5 u / 渭 l 3.0uq was dripped on both sides.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R-332

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