冈比亚按蚊触角气味结合蛋白基因AgOBP48表达载体的构建及在大肠杆菌中的表达
发布时间：2018-10-26 15:37
【摘要】：疟疾是严重危害人体健康的疾病之一，引起人类疟疾的所有疟原虫都是按蚊属昆虫传播的，，非洲冈比亚按蚊高度嗜吸人血，是恶性疟原虫极其高效的传播媒介，所以危害尤为严重，昆虫都是依靠嗅觉发现寄主的，嗅觉在按蚊的搜索寄主行为中起关键作用，昆虫能够感受空气中的挥发性气味分子，并依此作为寻偶、觅食和寻找产卵场所的信息。而昆虫触角感受器中的气味结合蛋白被认为是运输气味分子的受体，是昆虫专一性识别外界气味物质的第一步生化反应，对于昆虫与外界进行信息交流具有重要意义，本论文开展了对冈比亚按蚊的气味结合蛋白AgOBP48基因的研究。 利用英国洛桑研究所惠赠的含有AgOBP48基因的PET-20b(+)，用NdeⅠ／EcoRⅠ双酶切取得目的基因片段，将目的片断和原核表达载体pET-28a(+)经NdeⅠ／EcoRⅠ双酶酶切重组到表达型质粒中并转化入原核细胞中。表达序列测定结果表明：冈比亚按蚊气味结基因AgOBP48的成熟蛋白阅读框全长536bp，编码172个氨基酸残基，预测分子量和等电点分别为19kDa和5.00。 本研究将冈比亚按蚊AgOBP48基因整合入原核表达载体中表达，得到的重组蛋白在其N-端融合有His·Tag纯化标记，为下一步蛋白的纯化，功能研究奠定基础。
[Abstract]:Malaria is one of the diseases that seriously endanger human health, and all malaria parasites causing human malaria are transmitted by Anopheles mosquitoes, and the African Anopheles gambiae are highly blood-sucking and extremely efficient vectors of transmission of Plasmodium falciparum, So the damage is especially serious. Insects rely on smell to find their hosts. Smell plays a key role in the search for the host of Anopheles. Insects can sense volatile odour molecules in the air and use it as a match. Information about foraging and finding spawning places. The odor binding protein in the antennae is considered to be the receptor for the transport of odour molecules and the first step biochemical reaction for insect to specifically recognize the external odour substances. It is of great significance for the communication of information between insects and the outside world. In this paper, we studied the AgOBP48 gene of odour binding protein in Anopheles gambiae (Anopheles gambiae). The target gene fragment was obtained by using Nde 鈪
本文编号：2296193
[Abstract]:Malaria is one of the diseases that seriously endanger human health, and all malaria parasites causing human malaria are transmitted by Anopheles mosquitoes, and the African Anopheles gambiae are highly blood-sucking and extremely efficient vectors of transmission of Plasmodium falciparum, So the damage is especially serious. Insects rely on smell to find their hosts. Smell plays a key role in the search for the host of Anopheles. Insects can sense volatile odour molecules in the air and use it as a match. Information about foraging and finding spawning places. The odor binding protein in the antennae is considered to be the receptor for the transport of odour molecules and the first step biochemical reaction for insect to specifically recognize the external odour substances. It is of great significance for the communication of information between insects and the outside world. In this paper, we studied the AgOBP48 gene of odour binding protein in Anopheles gambiae (Anopheles gambiae). The target gene fragment was obtained by using Nde 鈪
本文编号：2296193
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