胰腺淋巴管的分布结构特点及其与胰岛素转运的关系

发布时间：2018-11-05 11:48

【摘要】： 目前,对于胰岛素的细胞内合成过程,已经有了比较清楚的认识,其中各个环节,已基本阐明。但是,对于胰岛素由B细胞分泌入组织间隙后的正常转运途径,深入的研究报道较少。目前所公认的途径为进入毛细血管,通过血液转运,并且已经有实验所证明。在生理状况下,除了通过血管系统之外,胰岛素是否还存在其他的转运途径?目前还存在争论。在上个世纪,许多学者报道了在不同实验对象的胸导管淋巴液中存在胰岛素,这就提示,胰岛素由B细胞分泌入组织液后,胰腺的淋巴系统,可能是生理状态下胰岛素的重要转运途径。如果这条途径存在,则具有重要的生理及病理意义:胰岛素通过淋巴系统转运,可不进入肝门静脉系统,避免了在肝脏的“首过效应”,胰岛素直接进入体循环,运达全身各处靶器官发挥作用;某些病理改变引起的胰腺淋巴回流障碍,导致胰岛素的淋巴途径转运受阻,可能会引起机体糖代谢的异常;对于其他种类多肽激素的转运,也有类比意义。本研究探讨了胰腺淋巴管的分布,胰岛素的细胞外转运途径,以期确定在生理状况下,是否存在淋巴转运途径,也涉及了淋巴回流受阻的情况下,对动物血糖水平的影响。实验内容和结果如下: 一、常用啮齿类实验动物胰腺淋巴管的分布和结构特点目的:由于研究手段和所选实验动物的差异,对于胰腺淋巴管的形态分布及其结构特点的认识,一直存在分歧。目前普遍认为,胰腺小叶间结缔组织内存在集合淋巴管,并且与胰腺的血管及外分泌导管并行;胰岛内部仅存在毛细血管,无淋巴管分布。对于胰腺小叶内部是否分布有淋巴管,实验结论不尽相同,有实验研究证实,在胰腺小叶内部,存在丰富的毛细淋巴管;也有学者观察后认为,小叶内缺乏或不存在毛细淋巴管。对于这个情况,有学者提出胰腺淋巴管的分布可能具有种属差异的观点,但实验依据并不充分。本研究拟对常用的啮齿类实验动物的胰腺淋巴管分布,进行详细研究,并在此基础上,观察胰腺淋巴管的超微结构特征,以及淋巴管与胰岛的关系。以期为
[Abstract]:At present, there has been a clear understanding of the intracellular synthesis of insulin, in which each link has been basically clarified. However, there are few reports on the normal translocation of insulin from B cells into the tissue space. The current recognized pathway is to enter the capillaries, transport through the blood, and has been demonstrated by experiments. Are there other pathways to insulin transport in physiological conditions other than through the vascular system? There are still arguments. In the last century, many researchers have reported the presence of insulin in the lymphatic fluid of thoracic ducts in different subjects, suggesting that the pancreatic lymphatic system after insulin is secreted by B cells into tissue fluid. It may be an important transport pathway of insulin in physiological state. If this pathway exists, it has important physiological and pathological significance: insulin is transported through the lymphoid system, which does not enter the hepatic portal system, avoids the "first-pass effect" in the liver, and insulin goes directly into the systemic circulation. To reach all the target organs throughout the body to play a role; Some pathological changes caused by pancreatic lymphatic return disturbance, leading to the lymphatic pathway of insulin is blocked, may lead to abnormal glucose metabolism, but also for other kinds of polypeptide hormone transport, also has analogical significance. In this study, the distribution of pancreatic lymphatic vessels and the extracellular transport pathway of insulin were studied in order to determine whether there were lymphatic transport pathways under physiological conditions and the effect of lymphatic return obstruction on blood glucose levels in animals. The contents and results of the experiment are as follows: 1. The distribution and structural characteristics of pancreatic lymphatic vessels in common rodent experimental animals. There has been disagreement about the morphological distribution and structural characteristics of pancreatic lymphatic vessels. It is generally believed that there are aggregate lymphatic vessels in connective tissue of the pancreas, which are parallel to the vessels and exocrine ducts of the pancreas, and there are only capillaries in the islets without lymphatic vessels. As to whether there are lymphatic vessels in the pancreatic lobules, the experimental results are different. Some experimental studies have confirmed that there are abundant lymphatic capillaries in the pancreatic lobules. Some scholars also observed that there were no or no capillary lymphatics in the lobules. In this case, some scholars suggest that the distribution of pancreatic lymphatic vessels may have different species, but the experimental basis is not sufficient. In this study, the distribution of pancreatic lymphatic vessels in common rodent experimental animals was studied in detail, and the ultrastructural characteristics of pancreatic lymphatic vessels and the relationship between lymphatic vessels and pancreatic islets were observed. With a view to
【学位授予单位】：河北医科大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R322

【参考文献】