O型口蹄疫病毒VP1蛋白的比较与牛O型表位肽基因工程疫苗的研制
发布时间：2018-11-05 12:34
【摘要】： 口蹄疫(Foot and mouth disease, FMD)是由口蹄疫病毒(Foot and mouth disease virus, FMDV)引起的主要侵害猪、牛等偶蹄动物的高度接触传染性疾病。在FMDV七个血清型中,O型是发现最早并流行最广的型别之一。目前对其免疫控制的主要手段是灭活疫苗的接种,但有一定局限性,如成本高、接种量大及有散发活毒的危险。为此,我们钓取了O型FMDV流行株VP1的编码基因,并结合中国及周边国家病毒株序列的比较分析,在此基础上,设计、构建了两株牛O型口蹄疫表位肽基因工程疫苗,并对其免疫效果进行了检测。 结果表明:一FMDV流行株的VP1基因与猪O型FMDV病毒株O/HKN/3/75的氨基酸同源性高达98%;O型FMDV西藏株(泛亚)VP1蛋白的RGD基序和G-H环的空间位置与文献所报道的一致;构建的新疆株牛O型蛋白疫苗和西藏株牛O型蛋白疫苗与猪O型FMDV疫苗免疫的阳性血清存在交叉识别;西藏株牛O型蛋白疫苗可在豚鼠诱生出西藏株口蹄疫病毒的特异性抗体。 本研究为研制更为有效的牛O型口蹄疫基因工程疫苗提供了可参考的实验依据。
[Abstract]:Foot-and-mouth disease (Foot and mouth disease, FMD) is a highly contagious disease caused by foot-and-mouth disease virus (Foot and mouth disease virus, FMDV), which mainly infects pigs, cattle and other cloven-hoofed animals. Among the seven serotypes of FMDV, type O is one of the earliest and most prevalent serotypes. At present, the main method of immunization control is inactivated vaccine, but it has some limitations, such as high cost, large amount of inoculation and risk of sporadic live virus. For this reason, we have isolated the coding gene of VP1 of type O FMDV epidemic strain, and combined with the comparative analysis of the sequence of virus strains in China and other countries, we have designed and constructed two strains of bovine foot-and-mouth disease epitope peptide gene engineering vaccine. The immune effect was tested. The results showed that the amino acid homology between the VP1 gene of a FMDV epidemic strain and O/HKN/3/75 of porcine type O FMDV virus was as high as 98%. The RGD motif and G-H ring of VP1 protein of O type FMDV Tibetan strain were consistent with those reported in the literature. The positive sera of Xinjiang bovine type O protein vaccine and Tibetan cattle type O protein vaccine immunized with porcine type O FMDV vaccine were cross-recognized, and Tibetan bovine type O protein vaccine could induce specific antibodies against the FMDV in guinea pigs. This study provides a reference experimental basis for the development of a more effective genetic engineering vaccine for bovine foot-and-mouth disease.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392
本文编号：2312099
[Abstract]:Foot-and-mouth disease (Foot and mouth disease, FMD) is a highly contagious disease caused by foot-and-mouth disease virus (Foot and mouth disease virus, FMDV), which mainly infects pigs, cattle and other cloven-hoofed animals. Among the seven serotypes of FMDV, type O is one of the earliest and most prevalent serotypes. At present, the main method of immunization control is inactivated vaccine, but it has some limitations, such as high cost, large amount of inoculation and risk of sporadic live virus. For this reason, we have isolated the coding gene of VP1 of type O FMDV epidemic strain, and combined with the comparative analysis of the sequence of virus strains in China and other countries, we have designed and constructed two strains of bovine foot-and-mouth disease epitope peptide gene engineering vaccine. The immune effect was tested. The results showed that the amino acid homology between the VP1 gene of a FMDV epidemic strain and O/HKN/3/75 of porcine type O FMDV virus was as high as 98%. The RGD motif and G-H ring of VP1 protein of O type FMDV Tibetan strain were consistent with those reported in the literature. The positive sera of Xinjiang bovine type O protein vaccine and Tibetan cattle type O protein vaccine immunized with porcine type O FMDV vaccine were cross-recognized, and Tibetan bovine type O protein vaccine could induce specific antibodies against the FMDV in guinea pigs. This study provides a reference experimental basis for the development of a more effective genetic engineering vaccine for bovine foot-and-mouth disease.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392
【参考文献】
相关期刊论文 前2条
1 独军政,常惠芸,丛国正,林彤,邵军军,魏小娟,刘在新,谢庆阁;Asia1型口蹄疫病毒VP1基因的克隆、原核表达及纯化[J];畜牧兽医学报;2005年06期
2 刘光清,刘在新,谢庆阁;口蹄疫病毒持续性感染形成原因的探讨[J];中国兽医科技;2003年04期
，本文编号：2312099
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