不同年龄人群粪便样本中克罗诺杆菌分离情况的研究

发布时间：2018-11-05 20:31

【摘要】：目的初步了解不同年龄人群可能感染克罗诺杆菌的情况,并比较从粪便样本中分离培养和鉴定克罗诺杆菌的方法。方法采用直接涂板和增菌后涂板的两种分离培养方法,生化实验和荧光定量PCR进行进一步鉴定,分种则用基因fusA序列分析的方法实现。结果本研究在1 285份不同年龄人群的粪便样本中分离得到克罗诺杆菌27株,阳性率为2.10%。各年龄组的阳性率,经过χ2检验差异无统计学意义。27株克罗诺杆菌分为4个种,主要为阪崎克罗诺杆菌和丙二酸盐克罗诺杆菌。用CSB增菌液和CCI选择性培养基要比用mLST/Van增菌液和EIA选择性培养基敏感性高(P0.05)。结论克罗诺杆菌对不同年龄组的人群都有潜在的危害性,但主要为婴幼儿,因此要加强监测,提高防范意识。
[Abstract]:Objective to investigate the possible infection of Crotonella in different age groups and compare the methods of isolation, culture and identification from fecal samples. Methods two methods of isolation and culture were used, biochemical experiment and fluorescence quantitative PCR were used to identify the two methods of direct coating and post-inoculation, and the method of gene fusA sequence analysis was used to separate the species. Results in this study, 27 strains of Croix were isolated from 1 285 fecal samples of different age groups, and the positive rate was 2. 10%. There was no significant difference in the positive rate of each age group by 蠂 2 test. 27 strains of Crotonia were divided into 4 species, mainly Hanshizaki and Crotonia malonate. The sensitivity of CSB medium and CCI medium was higher than that of mLST/Van medium and EIA medium (P0.05). Conclusion Clostridium clonorrhoeae is potentially harmful to the population of different age groups, but it is mainly infantile, so we should strengthen the surveillance and raise the awareness of prevention.
【作者单位】：中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室;复旦大学附属儿科医院微生物实验室;上海市疾病预防控制中心微生物室;
【基金】：艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2012ZX10004215)
【分类号】：R378

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