胰岛素样生长因子-1对甲状旁腺激素介导成骨细胞增殖及成骨活性的影响

发布时间：2018-11-11 18:07

【摘要】：目的通过构建乳鼠颅盖骨成骨细胞分离培养与鉴定方法,研究胰岛素样生长因子-1(Insulin-like growth factor-1,IGF-1)及IGF-1联合重组人甲状旁腺激素1-34(recombinant human parathyroid hormone 1-34,rhPTH1-34)对成骨细胞增殖及I型胶原蛋白mRNA表达的影响。方法培养乳鼠成骨细胞,并观察其形态及功能,以原代培养乳鼠成骨细胞为实验模型,分空白组、PTH组、IGF-1组及不同浓度IGF-1作用的PTH介导的成骨细胞组(0、10、50、100 ng/L)。通过不同剂量的胰岛素样生长因子-1(0、10、50、100 ng/L)联合10-9mol/L重组人甲状旁腺素刺激体外培养的成骨细胞,用噻唑蓝(MTT)法检测细胞的增殖能力,RT-PCR法检测成骨细胞I型胶原蛋白mRNA的表达。结果 PTH和IGF-1均可促进成骨细胞增殖;IGF-1联合PTH可以促进成骨细胞增殖,且具有剂量依赖性。PTH联合IGF-1使成骨细胞增殖能力增强、I型胶原蛋白mRNA表达增强。IGF-1可以促进成骨细胞I型胶原蛋白mRNA的表达,且具有剂量依赖性。IGF-1可以促进成骨细胞I型胶原蛋白mRNA的表达,且具有剂量依赖性。结论 PTH与IGF-1均可刺激成骨细胞增殖和分化,IGF-1可促进PTH对成骨细胞的分化、增殖。两者合用其作用增强,有协同促进作用。
[Abstract]:Objective to study the combination of insulin-like growth factor-1 (Insulin-like growth factor-1,IGF-1) and IGF-1 with recombinant human parathyroid hormone 1-34 (recombinant human parathyroid hormone 1-34 by constructing a method for isolation, culture and identification of osteoblasts from calvaria of newborn rats. The effect of rhPTH1-34 on the proliferation of osteoblasts and the expression of type I collagen mRNA. Methods Rat osteoblasts were cultured, and their morphology and function were observed. The primary cultured rat osteoblasts were divided into blank group, PTH group, IGF-1 group and PTH mediated osteoblast group (0101050100 ng/L). Osteoblasts cultured in vitro were stimulated with different doses of insulin-like growth factor-1 (010 ng/L) combined with recombinant human parathyroid hormone (10-9mol/L). The proliferation of osteoblasts was determined by thiazolyl (MTT) assay. The expression of type I collagen mRNA in osteoblasts was detected by RT-PCR. Results both PTH and IGF-1 could promote the proliferation of osteoblasts. IGF-1 combined with PTH could promote the proliferation of osteoblasts in a dose-dependent manner. PTH combined with IGF-1 enhanced the proliferation of osteoblasts. IGF-1 could promote the expression of type I collagen mRNA in osteoblasts in a dose-dependent manner, and IGF-1 could promote the expression of type I collagen mRNA in a dose-dependent manner. Conclusion both PTH and IGF-1 can stimulate the proliferation and differentiation of osteoblasts. IGF-1 can promote the differentiation and proliferation of osteoblasts induced by PTH. The combination of the two plays an enhanced role and has a synergistic effect.
【作者单位】：山西医科大学第一医院内分泌科;
【基金】：山西省科技厅科技发展计划项目(20130313022-4)
【分类号】：R329.2

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