大鼠异体肾移植动物模型的建立及术后外周血细胞因子的动态监测

发布时间：2018-11-11 18:19

【摘要】：目的:建立新疆地区第一个大鼠异体肾移植动物模型,为肾移植基础研究提供良好的技术平台。在成功建模的基础上,观察异体肾移植急性排斥大鼠术后外周血中IL-2、IL-4和IFN-γ的含量变化并探讨其意义。方法:采用远交系Wistar和SD大鼠,血管吻合采用供受体腹主动脉和下腔静脉端-侧连续缝合,尿路重建采用供受体输尿管端-端吻合,建立大鼠异体肾移植模型,并观察和比较Wistar-Wistar与SD-Wistar两组大鼠间异体肾移植后的存活情况,术后并发症及肾脏病理损害。把制备的大鼠模型分为四组,即正常对照组、假手术组、同系移植组和异系移植组,分批采集假手术组、正常对照组和同系及异系移植组大鼠在术后3d、5d、7d的外周血,监测鼠外周血中IL-2、IL-4、IFN-γ、Cr和BUN的动态改变,同时行移植肾病理检查。结果:大鼠异体肾移植手术时间平均为108±15min,手术成功率为83.33%(25/30)。对术后急性排斥大鼠体内的细胞因子的监测表明,IL-2、IFN-γ在急排早期明显升高,随后缓慢下降,IL-4虽有升高但比较正常对照组无统计学差异(P0.05)。发生急性排斥反应时,IL-2、IFN-γ的升高和移植肾病理损害程度无相关关系。Cr、BUN的变化和移植肾病理损害程度呈正相关(P0.05)。结论:本组大鼠异体肾移植动物模型成功率较高,并发症发生率较低,能满足实验研究的需要。急性排斥反应发生时,大鼠外周血中IL-2、IFN-γ的升高有助于早期诊断急性排斥反应,其升高水平与移植肾急性排斥病变程度无相关关系。在本研究中尚无证据支持IL-4的含量变化可以作为早期诊断急性排斥反应的依据。
[Abstract]:Aim: to establish the first animal model of allogeneic kidney transplantation in Xinjiang, and to provide a good technical platform for the basic research of renal transplantation. On the basis of successful modeling, the changes of IL-2,IL-4 and IFN- 纬 levels in peripheral blood of acute rejection rats after allograft transplantation were observed and their significance was discussed. Methods: a rat model of allograft kidney transplantation was established by end-to-side anastomosis of donor abdominal aorta and inferior vena cava and end-to-end ureteral anastomosis for urinary tract reconstruction in distal Wistar and SD rats. The survival, postoperative complications and renal pathological damage were observed and compared between Wistar-Wistar group and SD-Wistar group. The rat models were divided into four groups: normal control group, sham operation group, homologous transplantation group and allogeneic transplantation group, batch collection of sham operation group, normal control group, homologous group and allogeneic transplantation group. The dynamic changes of IL-2,IL-4,IFN- 纬, Cr and BUN in peripheral blood of rats were monitored. Results: the average time of allograft kidney transplantation was 108 卤15 min, and the success rate was 83.33% (25 / 30). The monitoring of cytokines in acute rejection rats after operation showed that IL-2,IFN- 纬 increased significantly at the early stage of acute rejection, and then decreased slowly. Although IL-4 was increased, there was no statistical difference compared with the normal control group (P0.05). When acute rejection occurred, there was no correlation between the increase of IL-2,IFN- 纬 and the degree of pathological damage of transplanted nephropathy. The change of Cr,BUN was positively correlated with the degree of pathological damage of transplanted nephropathy (P0.05). Conclusion: the success rate of allogeneic kidney transplantation in this group is high, and the incidence of complications is low, which can meet the need of experimental study. When acute rejection occurs, the increase of IL-2,IFN- 纬 in peripheral blood of rats is helpful to early diagnosis of acute rejection, and there is no correlation between the elevated level and the severity of acute rejection of transplanted kidney. There is no evidence in this study that changes in IL-4 levels can be used as a basis for early diagnosis of acute rejection.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R-332

【参考文献】