单克隆抗体电荷异构体分离方法优化
发布时间：2018-11-13 16:23
【摘要】：正单克隆抗体是复杂的四聚体糖蛋白,常呈现微观不均一性,即"异质性",包括电荷、疏水、形态等相关的异构体[1]。这些异构体可能来自于抗体分子复杂的生物合成途径,如细胞系及培养工艺[2],也可能来自于纯化、制剂等制造过程以及贮存过程的任何阶段[1]。其中由于抗体分子所带电荷差异造成的异质性称为电荷异构体,一般分为酸性异构体和碱性异构体,产生原因主要与翻译后修饰有关。电荷异
[Abstract]:Positive monoclonal antibodies are complex tetramer glycoproteins, which often exhibit microscopic heterogeneity, including charge, hydrophobicity, morphology and other related isomers [1]. These isomers may be derived from complex biosynthetic pathways of antibody molecules, such as cell lines and culture processes [2], or from any stage in the manufacturing process, such as purification, preparation, and storage [1]. The heterogeneity caused by the charge difference of antibody molecules is called charge isomers, which are generally divided into acidic isomers and alkaline isomers, which are mainly caused by post-translational modification. Charge heterogeneity
【作者单位】： 中国药科大学生命科学与技术学院;江苏先声药业有限公司;
【分类号】：R392
[Abstract]:Positive monoclonal antibodies are complex tetramer glycoproteins, which often exhibit microscopic heterogeneity, including charge, hydrophobicity, morphology and other related isomers [1]. These isomers may be derived from complex biosynthetic pathways of antibody molecules, such as cell lines and culture processes [2], or from any stage in the manufacturing process, such as purification, preparation, and storage [1]. The heterogeneity caused by the charge difference of antibody molecules is called charge isomers, which are generally divided into acidic isomers and alkaline isomers, which are mainly caused by post-translational modification. Charge heterogeneity
【作者单位】： 中国药科大学生命科学与技术学院;江苏先声药业有限公司;
【分类号】：R392
【共引文献】
相关期刊论文 前4条
1 许峰;章良;;利用多模式填料降低治疗用单克隆抗体中的酸性组分[J];广东化工;2014年10期
2 刘铁成;;离子交换色谱法在抗EGFR单克隆抗体检测中的应用[J];生物技术世界;2013年10期
3 郭玮;于传飞;李萌;王兰;张峰;刘春雨;王文波;高凯;;N端测序作为单克隆抗体常规放行分析方法的探讨[J];生物工程学报;2014年09期
4 刘_薏，
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