大鼠肝缺血再灌注损伤对肾的影响

发布时间：2018-11-16 07:44

【摘要】：目的：观察大鼠肝缺血再灌注(I／R)损伤过程对肾的影响并探讨其可能的机制。方法：建立肝缺血再灌注损伤动物模型，选健康Wistar大鼠48只，雌雄各半，按阻断血流后不同时间点分为六组，即对照组、缺血30min组(I组)、缺血30min再灌注组(I／R组)、缺血30min再灌注后1h组(I／R1h组)、缺血30min再灌注后2h组(I／R2h组)、缺血30min再灌注后4h组(I／R4h组)，每组8只。每组均测定血中谷氨酰转肽酶(GTP)、血清磷酸酶(AKP)、γ-谷氨酰氨基转移酶(γ-GT)、尿素氮(BUN)、肌酐(Cr)及肾组织中的丙二醛(MDA)的含量，并测定血浆和肾组织血管紧张素Ⅱ(AngⅡ)、醛固酮(Ald)、内皮素(ET)的含量。结果：肝脏I／R导致肝脏明显的损伤，表现为血清GTP在I组与对照组相比明显升高，差异有统计学意义(P＜0.05)；I／R、I／R1h、I／R2h、I／R4h组与I组比较，差异有统计学意义(P＜0.05)；I／R1h、I／R2h、I／R4h组与I／R组比较，差异有统计学意义(P＜0.05)；I／R2h、I／R4h组与I／R1h组比较差异有统计学意义(P＜0.05)；I／R4h组与I／R2h组相比，差异有统计学意义(P＜0.05)。AKP在I组与对照组相比明显升高，差异有统计学意义(P＜0.05)；I／R、I／R1h、I／R2h、I／R4h组与I组比较，，差异有统计学意义(P＜0.05)。γ-GT的含量也有升高的趋势，I／R1h、I／R2h、I／R4h组与对照组、I、I／R组比较，差异有统计学意义(P＜0.05)。随着肝I／R时间的延长，肝脏结构出现了明显改变，表现为炎性细胞浸润，肝细胞片状坏死。血中BUN含量随再灌注时间延长逐渐升高，I／R2h、I／R4h组与对照组、I、I／R、I／R1h组相比明显升高，差异有统计学意义(P＜0.05)；I／R4h组与I／R2h组相比，差异有统计学意义(P＜0.05)。血中Cr以及肾组
[Abstract]:Aim: to observe the effect of hepatic ischemia reperfusion (I / R) injury on the kidney and to explore its possible mechanism. Methods: the animal model of hepatic ischemia-reperfusion injury was established. 48 healthy Wistar rats, half male and half female, were divided into six groups according to different time points after occlusion: control group, ischemic 30min group (group I) and ischemic 30min reperfusion group (I / R group). One hour after 30min reperfusion (I/R1h group), two hours after ischemia 30min reperfusion (I/R2h group) and four hours after ischemia 30min reperfusion (I/R4h group), 8 rats in each group. The levels of serum (AKP), 纬 -glutamyl aminotransferase (AKP), 纬 -glutamyl aminotransferase) (AKP), 纬 -glutamyl aminotransferase) in serum and malondialdehyde (MDA) in renal tissue were measured in each group. The contents of angiotensin 鈪

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