STZ诱导小鼠糖尿病动物模型的机制探讨

发布时间：2018-11-16 06:47

【摘要】：目的:探讨不同剂量链脲佐菌素(STZ)诱导小鼠糖尿病模型的机制,寻找STZ 诱导1 型糖尿病模型的最佳剂量。方法:本研究采用多次重复注射不同剂量(20mg/kg·bw,40mg/kg·bw,80mg/kg·bw)的STZ 诱导昆明小鼠糖尿病模型;采用葡萄糖(Glu)测定试剂盒与尿液分析试纸条联合检测了小鼠血糖和尿糖的变化;采用常规HE 切片光镜下观察的方法检测胰岛的组织学改变;采用酶联免疫吸附试验(间接ELISA 法)测定小鼠血清中胰岛素自身抗体(IAA)的水平;采用淋巴细胞转化试验(MTT 法)测定小鼠胸腺和脾脏的T 淋巴细胞功能。结果:1.STZ 剂量选择:本研究应用20mg/kg·bw,40mg/kg·bw,80mg/kg·bw 多次注射STZ 诱导小鼠DM。2.体重:在注射STZ 后第三天Ⅳ组小鼠的体重明显下降,而Ⅱ组、Ⅲ组也较对照组轻度下降。随着时间的推移,Ⅳ组小鼠的体重不断下降,而Ⅱ组、Ⅲ组则随时间增长而体重增加,但与对照组相比Ⅱ组、Ⅲ组小鼠体重的增加是缓慢的。3. 血糖、尿糖:在最后一次注射STZ 后短期内(第4 天)Ⅳ组模型鼠的血糖即升高,而在第14 天～21 天血糖值开始下降;尿糖均++++。Ⅱ组、Ⅲ组模型鼠血糖虽在开始升高不明显,但1 周后的血糖值则随时间增长逐渐持续增加;尿糖Ⅱ组中有5 只为-,其余均+,Ⅲ组中有2 只为-,且均为雌性,其余均++。Ⅰ组小鼠血糖值随时间增长没有变化,尿糖均为-。4.胰腺组织学观察:正常组的胰岛为圆形、椭圆形或条索状细胞团,β细胞数量较多,胞质丰富,核圆形。Ⅱ组、Ⅲ组成模鼠的胰岛变小,胰岛细胞数量减少,可见以淋巴细胞、单核细胞为主体的炎细胞浸润,胰岛β细胞空泡变性、坏死、消失,使胰岛呈空虚状态;Ⅳ组小鼠偶见胰岛存在。5.自身抗体产生:在注射STZ 后短期内(第4 天)各组与对照组无明显差别;但随着时间的推移(第7 天起)Ⅱ组和Ⅲ组的自身抗体增加,而Ⅳ
[Abstract]:Aim: to investigate the mechanism of different doses of streptozotocin (STZ) induced diabetic model in mice and to find out the best dose of STZ induced type 1 diabetes. Methods: Kunming mice model of diabetes was induced by multiple repeated injection of different doses (20mg/kg bw,40mg/kg bw,80mg/kg bw) of STZ. Glucose (Glu) assay kit and urine analysis strip were used to detect the changes of blood glucose and urine sugar in mice, and the histological changes of pancreatic islets were detected under light microscope with routine HE sections. Enzyme linked immunosorbent assay (ELISA) was used to detect the level of insulin autoantibodies (IAA) in serum of mice, and lymphocyte transformation test (MTT) was used to detect the function of T lymphocytes in thymus and spleen of mice. Results: 1.STZ dose selection: in this study, mice DM.2. was induced by multiple injection of STZ with 20mg/kg bw,40mg/kg bw,80mg/kg bw. Body weight: on the third day after STZ injection, the body weight of group 鈪，

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