体重正常胰岛素抵抗大鼠模型制备与评价

发布时间：2018-11-19 11:46

【摘要】：目的：研究自行设计的高蔗糖饲料喂养SD大鼠制备正常体重胰岛素抵抗(IR)大鼠模型，并评价该饮食对大鼠IR的影响及可能机制。方法：30只雄性SD大鼠随机分为两组：正常对照(normal control，NC)组(n=15)，喂养普通饮食；试验组即高蔗糖(high-sucrose，HS)组，喂养高蔗糖饮食。实验期间每组给予相应的饲料喂养，每3天测量体重，喂养16周后行腹腔内注射葡萄糖耐量试验(IPGTT)。喂养20周后采空腹血样测量空腹血糖(FBG)、总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白(HDL-c)、游离脂肪酸(FFA)及胰岛素水平。并采用3-~3H标记葡萄糖作为示踪剂建立自由状态下大鼠扩展高胰岛素-正常血糖钳夹技术评价动物胰岛素敏感性。测定腹内脂肪重量。取肝脏、骨骼肌、胸主动脉做HE染色、肝脏苏丹Ⅲ染色光镜及电镜观察大鼠组织形态学变化。结果：HS组与NC组大鼠比较，体重无明显差异，HS组糖耐量减低，HS组TG、空腹胰岛素水平升高，，HDL-c下降，两组FBG、TC、FFA无明显差异。HS组钳夹稳态GIR明显降低，HS组GDR明显下降，HGO增加。表明本高蔗糖饮食可成功诱导大鼠产生外周及肝脏的IR。HS组腹内脂肪较NC组明显增多。将所有大鼠钳夹时的GDR与腹内脂肪重量作相关性分析，结果提示GDR与腹内脂肪重量呈负相关。病理观察，HS组大鼠肝脏
[Abstract]:Aim: to study the effect of high sucrose diet on insulin resistance (IR) rat model of normal body weight in SD rats, and to evaluate the effect of high sucrose diet on IR in rats and its possible mechanism. Methods: thirty male SD rats were randomly divided into two groups: normal control (normal control,NC) group (n = 15) and high sucrose (high-sucrose,HS) group. During the course of the experiment, each group was fed with corresponding feed. Body weight was measured every 3 days. After 16 weeks of feeding, intraperitoneal injection of glucose tolerance test (IPGTT).) was performed. Fasting blood samples were collected after 20 weeks of feeding. Fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-c), free fatty acid (FFA) and insulin were measured. In addition, 3-tri-3H labeled glucose was used as tracer to establish the expanded hyperinsulin-normal glucose clamp technique in free state to evaluate the insulin sensitivity of animals. The weight of abdominal fat was measured. The liver, skeletal muscle and thoracic aorta were stained with HE. The histological changes of rats were observed under light microscope and electron microscope. Results: there was no significant difference in body weight between HS group and NC group. In HS group, glucose tolerance was decreased, fasting insulin level of TG, in HS group was increased, HDL-c level was decreased, and FBG,TC,FFA was not significantly different between the two groups. GDR decreased and HGO increased in HS group. The results showed that the high sucrose diet could induce the abdominal fat of the IR.HS group which produced peripheral and liver were significantly higher than that of the NC group. The correlation analysis between GDR and abdominal fat weight in all rats showed that GDR was negatively correlated with abdominal fat weight. Pathological observation: HS group rats live
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332;R587.1

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