母胎界面L-选择素和其配体表达及对胚胎着床的影响
发布时间：2018-11-19 12:42
【摘要】：在生殖、免疫、炎症的一些关键性过程中,分子水平上存在相似的特征。黏附分子L-选择素与其配体的结合介导了免疫系统的淋巴细胞归巢,及炎症过程白细胞与内皮细胞的黏附,并发挥着信号分子作用。近年研究者发现,参与免疫和炎症过程的重要细胞黏附分子L-选择素及其配体也存在于哺乳动物着床期母胎界面上。Genbacev等发现L-选择素与其配体的结合可能构成人类胚胎着床的起始步骤。着床窗口期,胚胎表面的L-选择素和母体子[谀-选择素的寡糖配体似乎都呈现上调趋势。L-选择素配体至少包括四种分子:CD34、粘膜区素细胞粘附分子-1(MAdCAM-1)、依赖糖基化的细胞粘附分子-1(GlyCAM-1)、Sgp200;由于最早发现于小鼠淋巴结高内皮静脉,故将它们统称为周围淋巴结区素(PNAd)。L-选择素识别硫酸化形式的配体,N-乙酰葡萄糖胺-6-转磺酶(GlcNAc6ST)在功能型L-选择素配体生成过程中发挥重要作用。 尽管以往的研究发现了人类母胎界面上存在L-选择素和其配体,但有关早期胚胎发育过程中L-选择素的表达规律、周期性变化的子[谀ど螸-选择素配体表达的时间、空间模式、妊娠建立后它们又将如何变化、它们的表达水平是否与妊娠成功率有关,这一系列问题目前尚未见报道。尽管体外研究表明这对配体一受体确实能够介导细胞之间的黏附,但尚无体内实验证明它们在胚胎着床中的作用。为了深入探讨L-选择素系统在胚胎着床中的功能和影响,我们检测人类母胎界面L-选择素及其配体表达的时间规律、位置特征,分析分泌期子[谀-选择素配体(PNAd)各组成分子以及GlcNAc6ST mRNA水平与体外
[Abstract]:There are similar characteristics at the molecular level in some key processes of reproduction, immunity, and inflammation. The binding of the adhesion molecule L- selectin to its ligand mediates the homing of lymphocytes in the immune system and the adhesion of leukocytes to endothelial cells during inflammation and acts as a signaling molecule. In recent years, researchers have found that L- selectin and its ligands, which are important cell adhesion molecules involved in immune and inflammatory processes, also exist at mammalian maternal and fetal interfaces. Genbacev et al found that the binding of L- selectin to its ligands may constitute the initial step of human embryo implantation. L- selectin and mother on embryo surface during implantation window period [? Flat-selectin oligosaccharide ligands appear to be on an upward trend. Lselectin ligands contain at least four molecules: CD34, mucosal adhesion molecule-1 (MAdCAM-1), glycosylated cell-adhesion molecule-1 (GlyCAM-1), and glycosylated oligosaccharide ligands. Sgp200; Because they were first found in the high endothelial veins of the lymph nodes of mice, they were collectively referred to as the ligand of (PNAd). L- selectin recognition of sulfated form in peripheral lymph nodes. N-acetyl glucosamine-6-transferase (GlcNAc6ST) plays an important role in the formation of functional L-selectin ligands. Although previous studies have found the presence of L- selectin and its ligands at the human maternal and fetal interface, the expression of L- selectin during early embryonic development is related to the periodic variation of the L- selectin gene [? The time, spatial pattern, how they will change after pregnancy and whether their expression level is related to the success rate of pregnancy have not been reported. Although in vitro studies have shown that these ligands and receptors can indeed mediate cell adhesion, no in vivo studies have demonstrated their role in embryo implantation. In order to study the function and influence of L- selectin system in embryo implantation, we detected the expression of L- selectin and its ligand in human maternal and fetal interface, and analyzed the secretory phase of L- selectin and its ligand. Flat- selectin ligand (PNAd) components, levels of GlcNAc6ST mRNA and in vitro
【学位授予单位】：浙江大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R321
本文编号：2342347
[Abstract]:There are similar characteristics at the molecular level in some key processes of reproduction, immunity, and inflammation. The binding of the adhesion molecule L- selectin to its ligand mediates the homing of lymphocytes in the immune system and the adhesion of leukocytes to endothelial cells during inflammation and acts as a signaling molecule. In recent years, researchers have found that L- selectin and its ligands, which are important cell adhesion molecules involved in immune and inflammatory processes, also exist at mammalian maternal and fetal interfaces. Genbacev et al found that the binding of L- selectin to its ligands may constitute the initial step of human embryo implantation. L- selectin and mother on embryo surface during implantation window period [? Flat-selectin oligosaccharide ligands appear to be on an upward trend. Lselectin ligands contain at least four molecules: CD34, mucosal adhesion molecule-1 (MAdCAM-1), glycosylated cell-adhesion molecule-1 (GlyCAM-1), and glycosylated oligosaccharide ligands. Sgp200; Because they were first found in the high endothelial veins of the lymph nodes of mice, they were collectively referred to as the ligand of (PNAd). L- selectin recognition of sulfated form in peripheral lymph nodes. N-acetyl glucosamine-6-transferase (GlcNAc6ST) plays an important role in the formation of functional L-selectin ligands. Although previous studies have found the presence of L- selectin and its ligands at the human maternal and fetal interface, the expression of L- selectin during early embryonic development is related to the periodic variation of the L- selectin gene [? The time, spatial pattern, how they will change after pregnancy and whether their expression level is related to the success rate of pregnancy have not been reported. Although in vitro studies have shown that these ligands and receptors can indeed mediate cell adhesion, no in vivo studies have demonstrated their role in embryo implantation. In order to study the function and influence of L- selectin system in embryo implantation, we detected the expression of L- selectin and its ligand in human maternal and fetal interface, and analyzed the secretory phase of L- selectin and its ligand. Flat- selectin ligand (PNAd) components, levels of GlcNAc6ST mRNA and in vitro
【学位授予单位】：浙江大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R321
【参考文献】
相关期刊论文 前1条
1 范衡宇,白玉妍,杨增明;胚泡着床窗口的分子调控[J];生物化学与生物物理进展;2001年01期
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