慢性疲劳综合症大鼠模型的建立

发布时间：2018-11-20 20:03

【摘要】：目的采用拥挤和睡眠紊乱方法制作慢性疲劳综合症(CFS)动物模型,观察拥挤程度、生活空间大小对造模的影响。方法大鼠拥挤在不同的小饲养箱内,并采用噪音和昼夜颠倒干扰大鼠的正常睡眠,造模持续7周。观察大鼠精神状态、皮毛色泽并评分,每周测量体质量、摄食量,研究结束时进行力竭游泳实验。结果造模后,大鼠一般表现评分值明显升高,大鼠体质量、摄食量、力竭游泳时间降低。其中生活空间最小、拥挤程度最高组的大鼠症状最典型:精神萎靡不振,攻击性、对抗性行为消失;皮肤松弛,皮下无脂肪;全身毛发枯槁,稀疏脱落,造模7周时一般表现评分值达到5.6分,体质量、摄食量、力竭游泳时间降低最明显。结论采用拥挤和睡眠紊乱方法成功构建了CFS动物模型,并且拥挤程度越高、生活空间越小慢性疲劳综合症表现越明显。
[Abstract]:Objective to make (CFS) animal model of chronic fatigue syndrome by means of crowding and sleep disorder, and to observe the influence of crowded degree and living space on the model making. Methods the rats were crowded in different feeding boxes, and the noise and the reverse of day and night were used to disturb the normal sleep of the rats. The model lasted 7 weeks. The mental state, fur color and color of the rats were observed, and the body mass and food intake were measured weekly. The exhaustive swimming experiment was carried out at the end of the study. Results after modeling, the general performance score of rats was significantly increased, and the body weight, food intake and exhaustive swimming time were decreased. The symptoms of the rats in the group of minimal living space and the highest degree of overcrowding were as follows: mental depression, aggression, and the disappearance of confrontational behavior; The whole body hair withered, sparse shed, the general performance score value reached 5.6 points after 7 weeks of modeling, body mass, food intake, exhausted swimming time decreased most obviously. Conclusion the CFS animal model was successfully constructed by the methods of crowding and sleep disorder. The higher the crowded degree, the more obvious the symptom of chronic fatigue syndrome with smaller living space.
【作者单位】：广东省医学实验动物中心;
【基金】：广东省中医药局课题(No.20112099) 广东省科技计划项目(No.2012B060300021);广东省科技计划项目(No.2011B010500013)
【分类号】：R-332

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