不同工艺提取麻疯树籽油及其杀灭血吸虫尾蚴效果

发布时间：2018-11-24 18:57

【摘要】：目的观察不同工艺提取的麻疯树籽油杀灭血吸虫尾蚴的效果,以筛选制备麻疯树籽油的最佳工艺及配方。方法采用6种不同工艺提取的麻疯树籽油,进行杀灭血吸虫尾蚴即时接触试验。采用麻疯树籽油及其与不同添加剂制成的制剂,进行昆明小鼠血吸虫尾蚴即时接触感染试验,观察其抗血吸虫感染的减虫率。结果 95%乙醇回流、料液比1∶8、2 h提取工艺出油率达30.7%。即时接触试验6种麻疯树籽油30 min内将尾蚴全部杀灭。即时接触感染试验麻风树籽油原液减虫率达70.97%,添加苯甲酸苄酯减虫率为58.87%,单一添加月桂氮酮减虫率达77.42%,添加苯甲酸苄酯、邻苯二甲酸二丁酯、月桂氮酮减虫率达100%。结论 95%乙醇回流、料液比1∶8、2 h提取工艺为麻疯树籽油最佳提取工艺。麻疯树籽油有较好的血吸虫尾蚴杀灭作用。
[Abstract]:Objective to observe the effect of jatropha curcas seed oil (Jatropha curcas L.) on killing cercariae of Schistosoma japonicum, and to select the best technology and formula for preparation of jatropha curcas seed oil. Methods six kinds of jatropha curcas seed oil were used to kill Schistosoma japonicum cercariae. Using jatropha curcas seed oil and its preparation made with different additives, the infection rate of Schistosoma japonicum cercariae in Kunming mice was observed. Results 95% ethanol was refluxed and the ratio of material to liquid was 1: 8 for 2 h. All cercariae were killed by 6 kinds of Jatropha curcas seed oil in 30 min. In the instant contact test, the insect reduction rate of the seed oil solution was 70.97, that of benzyl benzoate was 58.87, that of lauryl azone alone was 77.42, that of benzyl benzoate and dibutyl phthalate were added, and that of benzyl benzoate and dibutyl phthalate were added. The worm reduction rate of lauryl azone reached 100. Conclusion 95% ethanol was refluxed and the ratio of material to liquid was 1: 8 for 2 h, which was the best extraction process for jatropha curcas seed oil. Jatropha curcas seed oil can kill Schistosoma japonicum cercariae.
【作者单位】：湖北省疾病预防控制中心;应用毒理湖北省重点实验室;
【基金】：国家科技支撑计划(2009BAI78B04)
【分类号】：R383.24

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