活体观察贝伐单抗对人骨肉瘤裸鼠移植瘤模型的作用

发布时间：2018-12-11 23:20

【摘要】：目的探讨贝伐单抗(Avastin)对人骨肉瘤143-B裸鼠移植瘤模型生长和血管生成的影响。方法将成功建立的红色荧光蛋白标记的人骨肉瘤143-B裸鼠原位种植模型随机分为3组:对照组(生理盐水)、低剂量贝伐单抗组(Avastin-L,2.0mg/kg)和高剂量贝伐单抗组(Avastin-H,5.0mg/kg),每组7只。贝伐单抗每次腹腔注射0.2ml,1周2次,持续3周;对照组给予等体积生理盐水。每隔3天记录各组体质量,每隔7天用荧光影像系统测量原位肿瘤大小并计算抑瘤率;采用免疫组化En Vision法检测各组肿瘤组织中的CD34表达并计算微血管密度(MVD);酶联免疫吸附法检测各组血浆和肿瘤组织的血管内皮生长因子(VEGF)的水平。结果 3组实验裸鼠体质量及肺部转移率的差异无统计学意义(P0.05);与对照组相比,Avastin-L组和Avastin-H组的肿瘤体积减小,MVD、血浆及肿瘤组织的VEGF水平降低,差异均有统计学意义(P0.05);Avastin-H组对上述指标的改善程度优于Avastin-L组(P0.05)。结论 Avastin对人骨肉瘤143-B肿瘤生长有抑制作用,同时可降低血管生成及VEGF水平,但对肺部转移无明显抑制作用。
[Abstract]:Objective to investigate the effects of bevacizumab (Avastin) on the growth and angiogenesis of human osteosarcoma 143-B xenografts in nude mice. Methods Human osteosarcoma 143-B nude mice were randomly divided into three groups: control group (normal saline) and low dose bevacizumab group (Avastin-L,). 2.0mg/kg and high dose bevacizumab (Avastin-H,5.0mg/kg) with 7 rats in each group. Bevacizumab was intraperitoneally injected with 0.2 ml / d twice a week for 3 weeks, while the control group was given normal saline of the same volume. The body mass of each group was recorded every 3 days, the size of tumor in situ was measured and the tumor inhibition rate was calculated by fluorescence imaging system every 7 days, the expression of CD34 in tumor tissues was detected by immunohistochemical En Vision method and the microvessel density (MVD);) was calculated. The levels of vascular endothelial growth factor (VEGF) in plasma and tumor tissue were detected by Elisa. Results there was no significant difference in body mass and lung metastasis rate among the three groups (P0.05). Compared with the control group, the tumor volume of Avastin-L group and Avastin-H group decreased, and the VEGF level of MVD, plasma and tumor tissue decreased, the difference was statistically significant (P0.05). The improvement of the above indexes in Avastin-H group was better than that in Avastin-L group (P0.05). Conclusion Avastin can inhibit the growth of human osteosarcoma 143-B, decrease angiogenesis and VEGF level, but have no inhibitory effect on lung metastasis.
【作者单位】：第二军医大学南京临床医学院南京军区南京总医院骨科;
【基金】：江苏省面上基金资助项目(BK2010461)
【分类号】：R738.1;R-332

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