新生鼠兴奋毒性脑损伤模型

发布时间：2019-01-01 08:10

【摘要】：目的通过该模型的建立，为新生儿兴奋毒性脑损伤神经保护的研究提供一种动物模型，从而为新生儿围生期脑损伤的临床干预探索新的实验方法。方法选取不同窝别生后五天的ICR种小鼠60只，随机分为实验组和对照组。实验组脑内注射神经兴奋毒素ibotenate(鹅膏蕈氨酸)后再随机分为A组和B组，对照组脑内注射生理盐水后也随机分为C组和D组。脑内注射后24h选取A、C组断头处死，，120h选取B、D组断头处死。取脑组织切片、染色后行组织学观察，并通过有损伤的病理切片数量计算各组脑组织的损伤直径。结果实验组平均脑损伤直径明显大于对照组，实验组平均脑损伤直径与对照组相比较具有显著的差异性(p＜0.01)，差别具有统计学意义。结论实验组与对照组相比除了机械性损伤外还能形成大脑皮层的萎缩和脑室周围白质区明显的囊性病变。该模型的成功建立能为进一步开展新生儿兴奋毒性脑损伤神经保护的研究奠定基础。
[Abstract]:Objective to provide an animal model for the study of neuroprotection of neonatal excitotoxic brain injury, and to explore a new experimental method for the clinical intervention of neonatal perinatal brain injury. Methods 60 ICR mice were randomly divided into experimental group and control group. The experimental group was randomly divided into group A and group B after intracerebral injection of neuroexcitotoxin ibotenate, and the control group was also randomly divided into group C and group D after intracerebral injection of normal saline. 24 h after intracerebral injection, head-cut death was performed in group A C and group B D at 120 h. Brain tissue sections were taken and histologically observed after staining. The injury diameters of brain tissues were calculated by the number of pathological sections. Results the mean brain injury diameter in the experimental group was significantly larger than that in the control group, and there was significant difference between the experimental group and the control group (p < 0.01). Conclusion compared with the control group, the experimental group can cause atrophy of cerebral cortex and obvious cystic lesions in periventricular white matter area in addition to mechanical injury. The successful establishment of the model can lay a foundation for further research on neuroprotection of excitotoxic brain injury in neonates.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R722.12;R-332

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