Micro-CT观测豚鼠听骨链解剖结构的基础研究

发布时间：2019-01-16 01:48

【摘要】：目的利用计算机断层扫描显微成像(Micro-Computed Tomography,Micro-CT)技术拍摄豚鼠听骨链,获得完整清晰的3D图像,精确测量听骨链各组成部分尺寸,探讨豚鼠听骨链解剖结构。方法断头处死豚鼠,沿颅中线剪开头部,取出听泡剥除后内侧壁,苦味酸甲醛溶液固定后,利用Micro-CT技术获取三维图像。应用Avizo建模重构获得三维模型,分割染色听骨链并测量各听小骨组成结构。结果得到空间视觉良好、可任意轴向旋转的豚鼠听骨链3D模型,局部结构清晰,可任意分割显示并测量内外部结构,砧锤复合体、砧锤复合体长脚、砧锤复合体短脚、镫骨底板、镫骨长度、锤骨柄的长度分别为:3960±222μm、1180±36μm、909±11μm;2111±58μm、2496±104μm、3417±58μm;砧锤复合体、镫骨底板的宽度和锤骨柄高度为:1089±71μm、902±13μm、1760±74μm;砧骨镫骨夹角、砧骨锤骨夹角分别为:108.58°±1.26°、122.46°±4.04°。结论采用Micro-CT采集了豚鼠听泡的三维图像,并通过Avizo软件重建,精确分割出豚鼠听骨链的解剖结构并进行了精确测量,为豚鼠听骨链可视化研究提供了清晰直观的观察方式,测量并统计分析了豚鼠听骨链各小骨生理数据,为以豚鼠为对象的进一步听力学研究提供了新的研究方法。
[Abstract]:Objective to obtain complete and clear 3D images of auditory ossicular chain of guinea pigs by computer tomography microscopy (Micro-Computed Tomography,Micro-CT), and to accurately measure the size of each component of auditory ossicular chain, and to explore the anatomical structure of auditory chain of guinea pig. Methods the guinea pigs were killed by decapitation, the head was cut off along the middle line of the cranium, the inner wall was removed and fixed with picric acid formaldehyde solution, and 3D images were obtained by Micro-CT technique. The three-dimensional model was obtained by Avizo modeling and reconstructed. The ossicular chain was stained and the structure of each auditory ossicle was measured. Results 3D model of auditory chain in guinea pigs with good spatial vision and rotatable axial direction was obtained. The local structure was clear, the internal and external structures could be displayed and measured at any time, the anvil hammer complex had long feet, the anvil hammer complex had short feet and the anvil hammer complex had short feet. The length of stapes floor, stapes and pedicle of malleus were 3960 卤222 渭 m, 1180 卤36 渭 m, 909 卤11 渭 m, respectively. The width and height of stapes base plate of anvil hammer complex and stapes base plate were 1089 卤71 渭 m, 902 卤13 渭 m, 1760 卤74 渭 m, and the angle between incus and incus was 108.58 掳卤1.26 掳and 122.46 掳卤4.04 掳, respectively, in 2111 卤58 渭 m, 2 496 卤104 渭 m, 108.58 掳卤1.26 掳and 122.46 掳卤4.04 掳, respectively. Conclusion 3D images of auditory vesicles of guinea pigs were collected by Micro-CT and reconstructed by Avizo software. The anatomical structure of auditory ossicular chains of guinea pigs was accurately segmented and measured, which provided a clear and visual observation method for visualization of auditory ossicles in guinea pigs. The physiological data of each small bone in the auditory bone chain of guinea pigs were measured and statistically analyzed, which provided a new method for the further study of audiology in guinea pigs.
【作者单位】：民航总医院;北京大学民航临床医学院耳鼻咽喉头颈外科;中国民用航空局民用航空医学中心;中国科学院高能物理研究所;
【基金】：国家自然基金(U1333132) 北京市自然基金(2122060) 中国民用航空局民航科技创新引导资金
【分类号】：R816.96;R322

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