人胎海马神经元组织形态与自分泌运动因子及其受体的表达和发育研究
发布时间：2019-01-20 19:52
【摘要】：目的:①观察胎儿海马组织形态的发育变化。②观察胎儿海马内神经元的分化、生长及发育规律。③研究自分泌运动因子(autocrine motility factor,AMF)及自分泌运动因子受体(autocrine motility factor receptor,AMFR)在胎儿海马中的表达和意义。④探讨自分泌运动因子及其受体与胎儿海马发育的关系。方法:本实验收集胎儿29例,胎龄为11W～新生儿,根据海马组织不同发育阶段变化特点,将胚胎标本分三组,第一组为11W～16W,7例;第二组为17W～28W,17例;第三组为29W～新生儿,5例。取海马中段,常规石蜡包埋和切片。分别进行以下处理:①采用HE染色观察胎儿海马一般组织形态的发育变化;②采用硫堇染色观察胎儿海马神经元内尼氏体的发育变化;③应用免疫组织化学SABC法检测神经元特异性烯醇化酶(neuron specific enolase,NSE)、AMF和AMFR的表达及变化规律。结果:①HE染色切片中,第11W～16W胎儿海马的外部形态逐渐建立。海马内细胞分布密集,多为小而圆的细胞,胞质少,染色深。第17W开始可明显辨别海马本部CA1、CA2、CA3及CA4各区及齿状回,并可区分出海马皮质的分子层、锥体细胞层和多形细胞层。锥体细胞层内可见少量锥体形细胞和大量圆形细胞。齿状回可区分分子层、颗粒细胞层和多形细胞层,其中颗粒细胞层内细胞多呈圆形或椭圆形。随胎龄增长,海马锥体细胞层内细胞密度逐渐减小,细胞由圆形逐渐分化为锥体形,以中等大小的锥体细胞为多见。第29W以后,海马锥体细胞层内神经元逐渐发育成熟,细胞体积进一步增大,细胞密度进一步减小。②硫堇染色结果显示,在16W以前胎儿海马内几乎看不到尼氏体;从17W开始,海马锥体细胞层少数细胞内出现尼氏体,随胎龄增加,神经元内尼氏体渐趋丰富,染色加深。③免疫组织化学染色显示,胎儿发育到第17W时,海马的锥体细胞层和齿状回的颗粒细胞层内出现NSE阳性细胞,多形细胞层和分子层也有散在分布。NSE阳性细胞的分化速度随层次和区域不同而呈现差异,在锥体
[Abstract]:Objective: 1 to observe the morphological changes of fetal hippocampal tissue, 2 to observe the differentiation, growth and development of fetal hippocampal neurons. 3 to study autocrine motor factor (autocrine motility factor,AMF) and autocrine motor factor receptor (autocrine motility factor receptor,). (4) to investigate the relationship between autocrine motor factor and its receptor and fetal hippocampal development. Methods: 29 fetuses (gestational age 11W ~ newborns) were collected and divided into three groups according to the changes of hippocampal tissue in different developmental stages: the first group was 11W ~ 16W (n = 7), the second group (n = 17) was 17W ~ 28W (n = 17), the second group (n = 17) was divided into three groups. The third group was 29 W ~ newborns, 5 cases. The middle part of hippocampus was taken and paraffin embedded and sectioned. The following treatments were carried out: (1) HE staining was used to observe the morphological changes of fetal hippocampal tissues, 2) thionine staining was used to observe the development of Nissl bodies in fetal hippocampal neurons. 3 the expression and changes of neuron-specific enolase (neuron specific enolase,NSE), AMF and AMFR were detected by immunohistochemical SABC method. Results: in the 1HE staining section, the outer shape of the hippocampus of the 11W~16W fetus was gradually established. Hippocampal cells are densely distributed, mostly small and round cells, with less cytoplasm and deep staining. At the beginning of 17W, the CA1,CA2,CA3 and CA4 regions and dentate gyrus of hippocampus could be distinguished, and the molecular layer, pyramidal cell layer and polymorphic cell layer of hippocampal cortex could be distinguished. A small number of pyramidal cells and a large number of round cells can be seen in the pyramidal cell layer. Dentate gyrus can distinguish molecular layer, granular cell layer and polymorphic cell layer, in which the cells in granular cell layer are round or oval. With the increase of gestational age, the density of cells in the pyramidal cell layer of hippocampus gradually decreased, and the cells gradually differentiated from round to pyramidal, most of which were medium size pyramidal cells. After 29W, neurons in the pyramidal layer of hippocampus matured gradually, cell volume further increased, cell density decreased further. 2 the results of thionine staining showed that almost no Nissl bodies could be seen in the hippocampus of the fetus before 16W. From 17W onwards, a few cells of hippocampal pyramidal cell layer appeared Nissl bodies. With the increase of gestational age, the neuronal Nissl bodies gradually enriched and the staining deepened. 3 Immunohistochemical staining showed that the fetus developed to 17W. NSE positive cells were found in the pyramidal cell layer and granular cell layer of dentate gyrus in the hippocampus, and scattered in the polymorphic cell layer and molecular layer. The differentiation rate of NSE positive cells varied with different levels and regions, and the differentiation rate was different in the pyramidal body.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R329
[Abstract]:Objective: 1 to observe the morphological changes of fetal hippocampal tissue, 2 to observe the differentiation, growth and development of fetal hippocampal neurons. 3 to study autocrine motor factor (autocrine motility factor,AMF) and autocrine motor factor receptor (autocrine motility factor receptor,). (4) to investigate the relationship between autocrine motor factor and its receptor and fetal hippocampal development. Methods: 29 fetuses (gestational age 11W ~ newborns) were collected and divided into three groups according to the changes of hippocampal tissue in different developmental stages: the first group was 11W ~ 16W (n = 7), the second group (n = 17) was 17W ~ 28W (n = 17), the second group (n = 17) was divided into three groups. The third group was 29 W ~ newborns, 5 cases. The middle part of hippocampus was taken and paraffin embedded and sectioned. The following treatments were carried out: (1) HE staining was used to observe the morphological changes of fetal hippocampal tissues, 2) thionine staining was used to observe the development of Nissl bodies in fetal hippocampal neurons. 3 the expression and changes of neuron-specific enolase (neuron specific enolase,NSE), AMF and AMFR were detected by immunohistochemical SABC method. Results: in the 1HE staining section, the outer shape of the hippocampus of the 11W~16W fetus was gradually established. Hippocampal cells are densely distributed, mostly small and round cells, with less cytoplasm and deep staining. At the beginning of 17W, the CA1,CA2,CA3 and CA4 regions and dentate gyrus of hippocampus could be distinguished, and the molecular layer, pyramidal cell layer and polymorphic cell layer of hippocampal cortex could be distinguished. A small number of pyramidal cells and a large number of round cells can be seen in the pyramidal cell layer. Dentate gyrus can distinguish molecular layer, granular cell layer and polymorphic cell layer, in which the cells in granular cell layer are round or oval. With the increase of gestational age, the density of cells in the pyramidal cell layer of hippocampus gradually decreased, and the cells gradually differentiated from round to pyramidal, most of which were medium size pyramidal cells. After 29W, neurons in the pyramidal layer of hippocampus matured gradually, cell volume further increased, cell density decreased further. 2 the results of thionine staining showed that almost no Nissl bodies could be seen in the hippocampus of the fetus before 16W. From 17W onwards, a few cells of hippocampal pyramidal cell layer appeared Nissl bodies. With the increase of gestational age, the neuronal Nissl bodies gradually enriched and the staining deepened. 3 Immunohistochemical staining showed that the fetus developed to 17W. NSE positive cells were found in the pyramidal cell layer and granular cell layer of dentate gyrus in the hippocampus, and scattered in the polymorphic cell layer and molecular layer. The differentiation rate of NSE positive cells varied with different levels and regions, and the differentiation rate was different in the pyramidal body.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R329
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