新型调节性树突状细胞diffDC对LPS刺激的独特反应性及相关机制研究

发布时间：2019-01-23 17:01

【摘要】：本课题研究了新型调节型DC亚群diffDC对LPS刺激即TLR4信号途径的独特反应性。主要包括LPS刺激前后diffDC的表型和细胞因子以及趋化因子IP-10分泌的变化,并对其变化的分子机制进行了初步探讨。实验结果表明,与LPS刺激后maDC的细胞因子产生相反,在LPS刺激前后diffDC表现出了独特的反应性细胞因子表达谱,即高表达IL—10和NO而低分泌IL—12,其机制可能是由于ERK1/2的高度磷酸化,同时NF κ Bp65大量入核,启动IL-10的大量分泌,而由于diffDC的p38磷酸化水平很低,由此低分泌IL-12p70。diffDC高表达IRF3,在LPS刺激后可能被高度活化,从而使IFN-β高分泌,进而高度活化STAT1,最终引起IP-10分泌水平增高。diffDC通过特殊的信号通路分泌独特的细胞因子和趋化因子,可能有助于其吸引和调节免疫效应细胞和炎性细胞,维持自身稳态。
[Abstract]:In this study, we investigated the unique reactivity of a novel regulatory DC subgroup diffDC to LPS stimulation, or TLR4 signaling pathway. The changes of diffDC phenotype and cytokines and the secretion of chemokine IP-10 before and after LPS stimulation were mainly included, and the molecular mechanism of the changes was also discussed. The results showed that, contrary to the cytokine production of maDC after LPS stimulation, diffDC showed a unique reactive cytokine expression profile before and after LPS stimulation, i.e. high expression of IL-10 and NO and low secretion of IL-12,. The mechanism may be due to the high phosphorylation of ERK1/2 and the entry of NF 魏 Bp65 into the nucleus to activate the secretion of IL-10, while the low level of p38 phosphorylation of diffDC leads to the low secretion of IRF3, by IL-12p70.diffDC. After stimulation of LPS, IFN- 尾 may be highly activated, and then highly activated STAT1, may lead to the increase of IP-10 secretion. DiffDC secretes unique cytokines and chemokines through a special signal pathway. It may help to attract and regulate immune effector cells and inflammatory cells and maintain self-homeostasis.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392

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