张应力诱导大鼠骨髓间充质干细胞骨向分化的实验研究
发布时间：2019-01-25 10:22
【摘要】：骨髓间充质干细胞(Mesenchymal stem cells,MSCs)是一类具有向中胚层、外胚层及内胚层多种组织细胞分化能力的原始细胞;在体外、体内均可定向分化为成骨细胞和成软骨细胞;作为这些骨生成细胞的来源细胞,MSCs是骨组织再生的细胞学基础。基于其多向分化潜能,骨髓MSCs目前已成为组织工程、细胞工程领域最重要的种子细胞,其潜在应用价值越来越受到人们重视;有关骨髓MSCs的基础和临床应用研究逐渐成为国际上干细胞领域研究的重点。 骨组织的再生和改建涉及复杂的力学刺激,其中机械力学刺激是其中非常重要的一环;尤其在牵张成骨(distraction osteogenesis,DO)的骨再生过程中,适宜的机械张应力刺激则显得尤为重要。在牵张成骨中,新骨形成涉及多种细胞参与,这包括未分化的间充质干细胞(MSCs)、成骨细胞(OB)及其前体细胞、成纤维细胞、破骨细胞、内皮细胞等;其中MSCs在机械张应力下向牵张区募集、增殖、及成骨细胞向分化,是新骨形成的细胞学基础。机械力学刺激是细胞在生物进化过程中受到的最基本的生物刺激之一。适当的机械力学刺激可以促进细胞的生长、分化和功能的维持,但过度或持续时间过长的力学刺激又会对细胞的结构和功能造成损害;因此细胞是如何感受力学刺激信号并将其转化为生物化学信号的,其中涉及哪些信号通路以及相关基因的表达,一直是各国学者不断探索的前沿课题。近年来,国内外学者
[Abstract]:Bone marrow mesenchymal stem cell (Mesenchymal stem cells,MSCs) is a kind of progenitor cells with the ability to differentiate into mesoderm, ectoderm and endoderm, which can be differentiated into osteoblasts and chondroblasts in vitro. As the source of these osteogenic cells, MSCs is the cytological basis for bone regeneration. Because of its multidirectional differentiation potential, bone marrow MSCs has become the most important seed cells in tissue engineering and cell engineering, and its potential application value has been paid more and more attention. The basic and clinical application of bone marrow MSCs has gradually become the focus of stem cell research in the world. The regeneration and remodeling of bone tissue involves complex mechanical stimulation, in which mechanical stimulation is a very important part, especially in the process of bone regeneration of distraction osteogenesis (distraction osteogenesis,DO). In distraction osteogenesis, new bone formation involves a variety of cell involvement, including undifferentiated mesenchymal stem cell (MSCs), osteoblast (OB) and its precursors, fibroblasts, osteoclasts, endothelial cells and so on. The recruitment, proliferation and differentiation of osteoblasts from MSCs to the distraction zone under mechanical tensile stress are the cytological basis of new bone formation. Mechanical stimulation is one of the most basic biological stimuli to cells during biological evolution. Proper mechanical stimulation can promote cell growth, differentiation and function maintenance, but excessive or prolonged mechanical stimulation will damage cell structure and function. Therefore, how cells perceive mechanical stimuli and convert them into biochemical signals, which signaling pathways and related genes are involved, has been a frontier topic for researchers all over the world. In recent years, scholars at home and abroad
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R329.2
本文编号：2415045
[Abstract]:Bone marrow mesenchymal stem cell (Mesenchymal stem cells,MSCs) is a kind of progenitor cells with the ability to differentiate into mesoderm, ectoderm and endoderm, which can be differentiated into osteoblasts and chondroblasts in vitro. As the source of these osteogenic cells, MSCs is the cytological basis for bone regeneration. Because of its multidirectional differentiation potential, bone marrow MSCs has become the most important seed cells in tissue engineering and cell engineering, and its potential application value has been paid more and more attention. The basic and clinical application of bone marrow MSCs has gradually become the focus of stem cell research in the world. The regeneration and remodeling of bone tissue involves complex mechanical stimulation, in which mechanical stimulation is a very important part, especially in the process of bone regeneration of distraction osteogenesis (distraction osteogenesis,DO). In distraction osteogenesis, new bone formation involves a variety of cell involvement, including undifferentiated mesenchymal stem cell (MSCs), osteoblast (OB) and its precursors, fibroblasts, osteoclasts, endothelial cells and so on. The recruitment, proliferation and differentiation of osteoblasts from MSCs to the distraction zone under mechanical tensile stress are the cytological basis of new bone formation. Mechanical stimulation is one of the most basic biological stimuli to cells during biological evolution. Proper mechanical stimulation can promote cell growth, differentiation and function maintenance, but excessive or prolonged mechanical stimulation will damage cell structure and function. Therefore, how cells perceive mechanical stimuli and convert them into biochemical signals, which signaling pathways and related genes are involved, has been a frontier topic for researchers all over the world. In recent years, scholars at home and abroad
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R329.2
【引证文献】
相关期刊论文 前1条
1 黎润光;邵景范;魏明发;;机械力学对体外骨髓间充质干细胞的影响[J];中国组织工程研究与临床康复;2007年03期
相关博士学位论文 前1条
1 杨丽;OP模鼠MSCs生物学特性及药物对大鼠MSCs骨向分化的干预研究[D];暨南大学;2007年
相关硕士学位论文 前2条
1 龚理;柚皮苷对骨质疏松模型大鼠MSCs增殖、骨向分化及凋亡影响的实验研究[D];暨南大学;2006年
2 郑晓黎;蛇床子素对大鼠骨髓间充质干细胞增殖和骨向分化的影响[D];暨南大学;2008年
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