人β-NGF基因载体的构建及其在兔骨髓间充质干细胞中的表达
发布时间：2019-01-24 19:40
【摘要】：神经生长因子(nerve growth factor,NGF)是在1952年由Levi-Montalcini等人发现的,并因此获得1986年诺贝尔生理学奖。NGF是最重要的神经营养因子之一,在神经、免疫、造血、生殖及内分泌等系统均有重要生物学作用,尤其在神经系统的作用已被公认。NGF对调节神经元的生长、发育、分化、存活及神经损伤的再生修复均具有重要作用,在Alzheimer's病、神经损伤等神经系统疾病中已证实有较好的疗效。NGF由三个亚基(α、β和γ)组成,NGF的生物学活性集中体现在β亚基上,因此又称为β-NGF。目前,NGF基因及其蛋白的生物学功能和临床应用研究仍是神经科学领域的重要课题之一。天然NGF可从表达丰富的动物组织中直接提取,但含量均相对较低,难以满足临床应用,直到近年来,人工重组NGF的问世,才基本解决了此问题。但因为NGF是大分子蛋白,不易透过血脑屏障,故治疗受到限制。因此,选择合适的给药方法是当前需要解决的重大课题,基因治疗方法是目前最有希望的方案之一。 目前,人们对用于细胞移植和基因治疗的供体细胞的研究较多,骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)是一群具有向成骨细胞,成脂肪细胞,肝脏细胞,心肌细胞和神经细胞等多种细胞分化的多潜能组织干细胞。由于它具有:①容易获取、培养;②可穿越血脑屏障,长期存活;③能有效地表达外源性基因;④移植后植入反应较弱等特点。因此,BMSCs成为细胞治疗和基因治疗的种子细胞,显示出广阔的应用前景。本研究的目的在于构建人β-NGF基因
[Abstract]:Nerve growth factor (nerve growth factor,NGF) was discovered by Levi-Montalcini et al in 1952 and won the 1986 Nobel Prize in Physiology. NGF is one of the most important neurotrophic factors in nerve, immunity and hematopoiesis. Reproductive and endocrine systems play important biological roles, especially in the nervous system. NGF plays an important role in regulating the growth, development, differentiation, survival and regeneration of nerve injury in Alzheimer's disease. NGF is composed of three subunits (伪, 尾 and 纬). The biological activity of NGF is concentrated on 尾 subunit, so it is also called 尾-NGF.. At present, the biological function and clinical application of NGF gene and its protein are still one of the important subjects in the field of neuroscience. Natural NGF can be extracted directly from animal tissues with abundant expression, but the content is relatively low, so it is difficult to meet the clinical application. In recent years, artificial recombinant NGF has not been able to solve this problem. However, treatment is limited because NGF is a macromolecular protein that does not easily penetrate the blood-brain barrier. Therefore, the choice of appropriate drug delivery method is an important issue to be solved. Gene therapy is one of the most promising methods. At present, there are many researches on donor cells used in cell transplantation and gene therapy. Bone marrow mesenchymal stem cells (bone marrow mesenchymal stem cells,BMSCs) are a group of osteoblasts, adipoblasts, liver cells. Multipotent tissue stem cells differentiated from cardiomyocytes and nerve cells. Because it is easy to obtain and culture, it can cross the blood-brain barrier and survive for a long time, it can express exogenous genes effectively, and the implantation response is weak after transplantation. Therefore, BMSCs is a seed cell for cell therapy and gene therapy. The aim of this study is to construct human 尾-NGF gene.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R346
[Abstract]:Nerve growth factor (nerve growth factor,NGF) was discovered by Levi-Montalcini et al in 1952 and won the 1986 Nobel Prize in Physiology. NGF is one of the most important neurotrophic factors in nerve, immunity and hematopoiesis. Reproductive and endocrine systems play important biological roles, especially in the nervous system. NGF plays an important role in regulating the growth, development, differentiation, survival and regeneration of nerve injury in Alzheimer's disease. NGF is composed of three subunits (伪, 尾 and 纬). The biological activity of NGF is concentrated on 尾 subunit, so it is also called 尾-NGF.. At present, the biological function and clinical application of NGF gene and its protein are still one of the important subjects in the field of neuroscience. Natural NGF can be extracted directly from animal tissues with abundant expression, but the content is relatively low, so it is difficult to meet the clinical application. In recent years, artificial recombinant NGF has not been able to solve this problem. However, treatment is limited because NGF is a macromolecular protein that does not easily penetrate the blood-brain barrier. Therefore, the choice of appropriate drug delivery method is an important issue to be solved. Gene therapy is one of the most promising methods. At present, there are many researches on donor cells used in cell transplantation and gene therapy. Bone marrow mesenchymal stem cells (bone marrow mesenchymal stem cells,BMSCs) are a group of osteoblasts, adipoblasts, liver cells. Multipotent tissue stem cells differentiated from cardiomyocytes and nerve cells. Because it is easy to obtain and culture, it can cross the blood-brain barrier and survive for a long time, it can express exogenous genes effectively, and the implantation response is weak after transplantation. Therefore, BMSCs is a seed cell for cell therapy and gene therapy. The aim of this study is to construct human 尾-NGF gene.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R346
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