腺嘌呤诱发大鼠慢性肾衰模型的病理学研究
发布时间：2019-02-10 19:47
【摘要】： 目的建立腺嘌呤诱发的大鼠慢性肾衰模型，探讨肾间质纤维化的发生与a-平滑肌肌动蛋白(a-SMA)表达的关系。 方法20只SD大鼠随机分为正常对照组和肾衰模型组，正常组以生理盐水10ml／kg／d灌胃；模型组以腺嘌呤250mg／kg／灌胃，共24天(第1～12天每日给药1次，第12天后隔日给药)。检测血清尿素氮、肌酐，，Ca和P含量，观察肾组织的病理改变和纤维的增生程度，免疫组化采用SP法观察肾组织中a-SMA和Ⅳ型胶原的表达。 结果与正常组比较模型组大鼠血清尿素氮和肌酐含量明显升高(p＜0.05)，Ca、P代谢失去平衡，肾小球数量明显减少，肾间质内大量纤维组织增生，大量a-SMA阳性细胞和Ⅳ型胶原在肾间质中分布。 结论腺嘌呤诱发的大鼠慢性肾衰模型是研究慢性肾衰和肾间质纤维化的较理想的动物模型，a-SMA阳性细胞在肾间质纤维化的发生过程中发挥重要的作用。
[Abstract]:Objective to establish a rat model of chronic renal failure induced by adenine and to investigate the relationship between the pathogenesis of renal interstitial fibrosis and the expression of a- smooth muscle actin (a-SMA). Methods Twenty SD rats were randomly divided into two groups: normal control group and renal failure model group. Normal group was given normal saline 10ml/kg/d. In the model group, adenine 250mg/kg/ was given orally for 24 days (once a day on the 12th day and every other day after the 12th day). The contents of serum urea nitrogen, creatinine, Ca and P, the pathological changes of renal tissue and the degree of fibrous hyperplasia were observed. The expression of a-SMA and type 鈪
本文编号：2419484
[Abstract]:Objective to establish a rat model of chronic renal failure induced by adenine and to investigate the relationship between the pathogenesis of renal interstitial fibrosis and the expression of a- smooth muscle actin (a-SMA). Methods Twenty SD rats were randomly divided into two groups: normal control group and renal failure model group. Normal group was given normal saline 10ml/kg/d. In the model group, adenine 250mg/kg/ was given orally for 24 days (once a day on the 12th day and every other day after the 12th day). The contents of serum urea nitrogen, creatinine, Ca and P, the pathological changes of renal tissue and the degree of fibrous hyperplasia were observed. The expression of a-SMA and type 鈪
本文编号：2419484
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