层粘连蛋白对人绒毛膜促性腺激素刺激下卵巢颗粒细胞孕酮分泌的影响
发布时间：2019-02-28 12:50
【摘要】： 目的:研究层粘连蛋白(LN)对人绒毛膜促性腺激素(hCG)刺激下大鼠排卵前颗粒细胞孕酮(P)分泌的影响及可能的作用机制。 方法:分离未成熟大鼠排卵前颗粒细胞,分3组进行培养:LN铺板组、未铺板组及LN铺板同时加入抗整合素α_6单抗(GoH3)组。观察比较细胞贴壁的情况。血清培养48h后加入hCG,无血清条件下再培养48h后收集培养液作孕酮测定及LH/hCG受体分析。 结果: LN铺板组颗粒细胞6小时即可贴壁及展开,而未铺板组16h开始贴壁及展开;无论hCG加入与否LN均可显著刺激颗粒细胞孕酮的产生, 5ug/mL的GoH3能明显抑制LN的此刺激作用。试验还观察到LN使颗粒细胞LH/hCG受体亲和力升高,受体数目上调,增强hCG刺激下颗粒细胞的孕酮分泌。 结论: LN通过与细胞表面粘附分子整合素α_6β_1(integrinα_6β_1)相互作用,可能为卵巢内的一种局部调节因素,干扰LH/hCG受体功能并增强颗粒细胞孕酮的产生,在围排卵期颗粒细胞黄素化过程中发挥重要效应。
[Abstract]:Aim: to study the effect of laminin (LN) on progesterone (P) secretion in rat granulosa cells stimulated by human chorionic gonadotropin (hCG) and its possible mechanism. Methods: pre-ovulatory granulosa cells were isolated from immature rats and cultured in three groups: LN group, LN group and anti-integrin 伪 _ 6 monoclonal antibody (GoH3) group. Observe and compare the cell adhesion. After 48 hours of serum culture, hCG, was added to the serum-free medium for 48 h and then collected for progesterone determination and LH/hCG receptor analysis. Results: the granulosa cells in the LN laminate group could adhere and expand in 6 hours, while those in the non-laminate group began to adhere and expand at 16 h. Whether hCG was added or not, LN could significantly stimulate progesterone production in granulosa cells, and GoH3 of 5ug/mL could significantly inhibit the stimulation of LN. It was also observed that LN increased the affinity of LH/hCG receptors and increased the number of receptors in granulosa cells, and enhanced the progesterone secretion of granulosa cells stimulated by hCG. Conclusion: through the interaction of LN with integrin 伪 _ 6 尾 _ 1 (integrin 伪 _ 6 尾 _ 1), it may be a local regulatory factor in ovary, interfere with the function of LH/hCG receptor and enhance the production of progesterone in granulosa cells. It plays an important role in the process of luteinization of granulosa cells during the peri-ovulatory period.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33
本文编号：2431811
[Abstract]:Aim: to study the effect of laminin (LN) on progesterone (P) secretion in rat granulosa cells stimulated by human chorionic gonadotropin (hCG) and its possible mechanism. Methods: pre-ovulatory granulosa cells were isolated from immature rats and cultured in three groups: LN group, LN group and anti-integrin 伪 _ 6 monoclonal antibody (GoH3) group. Observe and compare the cell adhesion. After 48 hours of serum culture, hCG, was added to the serum-free medium for 48 h and then collected for progesterone determination and LH/hCG receptor analysis. Results: the granulosa cells in the LN laminate group could adhere and expand in 6 hours, while those in the non-laminate group began to adhere and expand at 16 h. Whether hCG was added or not, LN could significantly stimulate progesterone production in granulosa cells, and GoH3 of 5ug/mL could significantly inhibit the stimulation of LN. It was also observed that LN increased the affinity of LH/hCG receptors and increased the number of receptors in granulosa cells, and enhanced the progesterone secretion of granulosa cells stimulated by hCG. Conclusion: through the interaction of LN with integrin 伪 _ 6 尾 _ 1 (integrin 伪 _ 6 尾 _ 1), it may be a local regulatory factor in ovary, interfere with the function of LH/hCG receptor and enhance the production of progesterone in granulosa cells. It plays an important role in the process of luteinization of granulosa cells during the peri-ovulatory period.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33
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本文编号：2431811
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