吗啡诱导大鼠穿梭觅药状态下内侧前额皮层边缘前区遥测脑电小波包提取及熵分析

发布时间：2019-03-12 16:21

【摘要】：本研究旨在研究内侧前额皮质(medial prefrontal cortex,m PFC)边缘前区(prelimbic cortical area,PL)遥测脑电的特征性改变与觅药行为之间的关系。将PL区埋藏脑立体定位电极的大鼠分成手术对照组和吗啡诱导组,后者制作吗啡诱导条件性位置偏爱(conditioned place preference,CPP)模型。利用CPP视频系统结合脑电无线遥测技术,记录两组大鼠在黑-白箱穿梭和白-黑箱穿梭时大鼠PL区实时脑电,采用小波包提取、Welch谱功率算法、归一化幅值和Shannon熵分析技术,对所测原始脑电波进行综合分析。结果显示,与手术对照组比较,吗啡诱导CPP组大鼠黑-白箱穿梭潜伏期,左侧PL区小波提取脑电各频段平均波幅减小,脑电成分在10~50 Hz频段Welch谱功率强度显著增加(P0.01或P0.05),脑电β、γ1、γ2波Shannon熵值升高(P0.01或P0.05),平均信息熵降低(P0.01)。上述结果提示,吗啡诱导CPP组大鼠黑-白箱穿梭时,左侧PL区脑电各频段大幅波减少,脑电10~50 Hz频率Welch谱功率强度增加,β、γ1和γ2波Shannon熵值升高;这些变化可能与大鼠觅药意识的形成和即将发动的觅药行为产生有关。
[Abstract]:The purpose of this study is to study the relationship between the characteristic change of the prefrontal cortex (pPFC) and the prefrontal cortex (PL) and the behavior of the drug. The rats were divided into the operative control group and the morphine induction group, which made the morphine-induced conditional position preference (CPP) model. The real-time EEG of the rat PL area was recorded by using the CPP video system in conjunction with the EEG wireless telemetry, and the real-time EEG of the rat PL area was recorded by using the wavelet packet extraction, the Welch spectrum power algorithm, the normalized amplitude and the Shannon entropy analysis technique in the black-white box shuttle and the white-black box. The measured original brain waves are comprehensively analyzed. The results showed that, compared with the control group, the average wave amplitude of the black-white box in the CPP group was reduced by the morphine-induced CPP group, and the average amplitude of the EEG in the left-side PL region was reduced, and the power intensity of the EEG in the 10-50 Hz frequency band was significantly increased (P0.01 or P0.05), and the EEG was 1, The entropy of the two-wave Shannon entropy increased (P0.01 or P0.05), and the average entropy of entropy decreased (P0.01). The results suggested that when morphine-induced CPP group was shuttled in the black-white box, the frequency band of the brain in the left-side PL region was significantly reduced, and the power intensity of the 10-50 Hz frequency Welch spectrum of the brain was increased, and the Shannon entropy value of 1 and 2 waves increased. These changes may be related to the formation of the consciousness of the drug administration in the rat and the impending drug action.
【作者单位】：皖南医学院计算机教研室;皖南医学院生理学教研室;
【基金】：supported by the Natural Science Foundation of Anhui Province,China(No.090413096)
【分类号】：R338

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