SARS冠状病毒Spike蛋白基因片段DNA疫苗及免疫效果研究
发布时间：2019-03-21 14:13
【摘要】：目的:严重急性呼吸道综合征(severe acute respiratorysyndrome, SARS)是一种急性呼吸道传染病,亦称为非典型肺炎。2002 年底,我国广东省首先出现了有记录SARS 病例,继而在2003 年初传播到世界30 多个国家,持续流行长达7个月,造成将近8100 人被感染,774 人死亡,死亡率超过9%。 SARS 的病原体已被确认为是一种新型冠状病毒,称为SARS 冠状病毒(SRAS-CoV)。毫无疑问,SRAS-CoV 给大众健康和社会经济造成了巨大的损失。虽然在SARS 第一次流行过后再罕有病例发生,但是,鉴于SARS 病毒具有多种传播途径和广泛的包括一些野生动物在内的非人类中间宿主,SARS 很有可能在将来再次暴发。目前,我们还没有针对SARS 的特效抗病毒药物或者疫苗。为了预防和控制今后SARS 的流行,研制针对SARS 冠状病毒的新型疫苗势在必行。 SARS-CoV 是基因组全长约30Kb 的单股正链RNA 病毒,属于冠状病毒属,和其他冠状病毒类似, SARS的病毒RNA基因组有5 个开放读码框架(ORF)和9 个潜在的开放读码框架,这些开放读码框架编码的蛋白包括RNA 聚合酶蛋白(polymerase protein)、刺突蛋白(spike protein, S)、小包膜蛋白(small membrane protein, E)、基质蛋白(matrix protein, M)和核衣壳蛋白(nucleocapsid protein, N)。在这些病毒蛋白中,
[Abstract]:Objective: severe acute respiratory syndrome (severe acute respiratorysyndrome, SARS) is an acute respiratory infectious disease, also known as atypical pneumonia. At the end of 2002, there were first recorded cases of SARS in Guangdong Province, China. The epidemic spread to more than 30 countries in the world in early 2003, lasting for seven months, leaving nearly 8100 people infected and 774 dead, with a mortality rate of more than 9 percent. The pathogen of SARS has been identified as a new type of coronavirus called SARS coronavirus (SRAS-CoV). There is no doubt that SRAS-CoV has caused enormous damage to public health and socio-economic. Although few cases have occurred after the first epidemic of SARS, SARS is likely to erupt again in the future, given that SARS has multiple transmission pathways and a wide range of non-human intermediate hosts, including some wild animals. At present, we do not have specific antiviral drugs or vaccines for SARS. In order to prevent and control the prevalence of SARS in the future, it is imperative to develop a new vaccine against SARS coronavirus. SARS-CoV is a single-stranded positive-stranded RNA virus with a total length of about 30Kb, belonging to the genus coronavirus, similar to other coronavirus. The RNA genome of SARS has five open reading frames (ORF) and nine potential open reading frames (ORF). These open reading frames encode proteins such as RNA polymerase protein (polymerase protein), spike protein (spike protein, S), small envelope protein (small membrane protein, E), matrix protein (matrix protein, M) and nucleocapsid protein (nucleocapsid protein, N). In these viral proteins,
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392
本文编号：2445015
[Abstract]:Objective: severe acute respiratory syndrome (severe acute respiratorysyndrome, SARS) is an acute respiratory infectious disease, also known as atypical pneumonia. At the end of 2002, there were first recorded cases of SARS in Guangdong Province, China. The epidemic spread to more than 30 countries in the world in early 2003, lasting for seven months, leaving nearly 8100 people infected and 774 dead, with a mortality rate of more than 9 percent. The pathogen of SARS has been identified as a new type of coronavirus called SARS coronavirus (SRAS-CoV). There is no doubt that SRAS-CoV has caused enormous damage to public health and socio-economic. Although few cases have occurred after the first epidemic of SARS, SARS is likely to erupt again in the future, given that SARS has multiple transmission pathways and a wide range of non-human intermediate hosts, including some wild animals. At present, we do not have specific antiviral drugs or vaccines for SARS. In order to prevent and control the prevalence of SARS in the future, it is imperative to develop a new vaccine against SARS coronavirus. SARS-CoV is a single-stranded positive-stranded RNA virus with a total length of about 30Kb, belonging to the genus coronavirus, similar to other coronavirus. The RNA genome of SARS has five open reading frames (ORF) and nine potential open reading frames (ORF). These open reading frames encode proteins such as RNA polymerase protein (polymerase protein), spike protein (spike protein, S), small envelope protein (small membrane protein, E), matrix protein (matrix protein, M) and nucleocapsid protein (nucleocapsid protein, N). In these viral proteins,
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392
【参考文献】
相关期刊论文 前1条
1 ;DNA Vaccine of SARS-Cov S Gene Induces Antibody Response in Mice[J];Acta Biochimica et Biophysica Sinica;2004年01期
，本文编号：2445015
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