钙激活钾通道及ATP敏感性钾通道在缺氧性肺血管收缩中的作用
发布时间：2019-04-22 15:03
【摘要】： 用半定量RT-PCR及Western blot方法观察缺氧对培养的大鼠肺动脉平滑肌细胞(pulmonary artery smooth muscle cells, PASMCs)上高电导钙激活钾通道(BKCa )及ATP敏感性钾通道Kir6.1和SUR2B基因表达及蛋白水平的影响。结果:(1)培养的PASMCs在基因水平和蛋白水平均存在BKCa及Kir6.1和SUR2B的表达。(2)急性缺氧6h在基因水平和蛋白水平均能上调BKCa及Kir6.1和SUR2B的表达。慢性缺氧48h时BKCa的基因水平和蛋白水平均上调,慢性缺氧72h时Kir6.1的基因水平和蛋白水平均上调。(3)慢性缺氧48h复氧12h再缺氧6h及慢性缺氧72h复氧12h再缺氧6h,BKCa及Kir6.1和SUR2B在基因水平和蛋白水平与正常组相比,均无显著性差异。结论:(1)BKCa及Kir6.1和SUR2B的基因水平和蛋白水平的表达在不同的缺氧时间上调,其可能在缺氧性肺血管收缩(hypoxic pulmonary vasoconstriction, HPV)中起到了负性调节作用,从而降低HPV。(2)慢性缺氧后再急性缺氧,BKCa及Kir6.1和SUR2B在基因水平和蛋白水平与正常组相比,均无显著性差异,这可能是机体的习服机制之一。
[Abstract]:Semi-quantitative RT-PCR and Western blot methods were used to observe the effect of hypoxia on (pulmonary artery smooth muscle cells, of cultured rat pulmonary artery smooth muscle cells The effects of high conductance calcium activated potassium channel (BKCa) and ATP sensitive potassium channel Kir6.1 and SUR2B gene expression and protein level on PASMCs). Results: (1) the expression of BKCa, Kir6.1 and SUR2B were found in cultured PASMCs at both gene and protein levels. (2) the expression of BKCa, Kir6.1 and SUR2B were up-regulated at gene and protein levels for 6 h after acute hypoxia. The gene and protein levels of BKCa were up-regulated at 48 h of chronic hypoxia and up-regulated at 72 h of chronic hypoxia. (3) the gene and protein levels of Kir6.1 were up-regulated at 48 h of chronic hypoxia and 12 h of reoxygenation for 12 h and 12 h of hypoxia for 12 h and 72 h of hypoxia for 12 h and 6 h, respectively. There was no significant difference in gene level and protein level of BKCa, Kir6.1 and SUR2B between normal group and control group. Conclusion: (1) the gene and protein levels of BKCa, Kir6.1 and SUR2B are up-regulated at different hypoxia time, which may play a negative role in hypoxic pulmonary vasoconstriction (hypoxic pulmonary vasoconstriction, HPV). There was no significant difference in gene and protein levels of BKCa, Kir6.1 and SUR2B between the two groups, which may be one of the mechanisms of acclimatization of HPV. (2).
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363
本文编号：2462937
[Abstract]:Semi-quantitative RT-PCR and Western blot methods were used to observe the effect of hypoxia on (pulmonary artery smooth muscle cells, of cultured rat pulmonary artery smooth muscle cells The effects of high conductance calcium activated potassium channel (BKCa) and ATP sensitive potassium channel Kir6.1 and SUR2B gene expression and protein level on PASMCs). Results: (1) the expression of BKCa, Kir6.1 and SUR2B were found in cultured PASMCs at both gene and protein levels. (2) the expression of BKCa, Kir6.1 and SUR2B were up-regulated at gene and protein levels for 6 h after acute hypoxia. The gene and protein levels of BKCa were up-regulated at 48 h of chronic hypoxia and up-regulated at 72 h of chronic hypoxia. (3) the gene and protein levels of Kir6.1 were up-regulated at 48 h of chronic hypoxia and 12 h of reoxygenation for 12 h and 12 h of hypoxia for 12 h and 72 h of hypoxia for 12 h and 6 h, respectively. There was no significant difference in gene level and protein level of BKCa, Kir6.1 and SUR2B between normal group and control group. Conclusion: (1) the gene and protein levels of BKCa, Kir6.1 and SUR2B are up-regulated at different hypoxia time, which may play a negative role in hypoxic pulmonary vasoconstriction (hypoxic pulmonary vasoconstriction, HPV). There was no significant difference in gene and protein levels of BKCa, Kir6.1 and SUR2B between the two groups, which may be one of the mechanisms of acclimatization of HPV. (2).
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363
【参考文献】
相关期刊论文 前1条
1 王国勇，，郑永芳，屈金河，王晓芳;SHRsp大鼠阻力血管平滑肌IKca对Ca~（2＋）i敏感性的改变[J];基础医学与临床;1994年03期
本文编号：2462937
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