大鼠缺血性结肠炎模型的建立及用PGE1、SF治疗效果的研究
发布时间：2019-04-22 19:09
【摘要】：目的:一、建立大鼠缺血性结肠炎模型并探讨其发病机制。 二、使用不同剂量的脂微球前列腺素E1(Lipo-PGE1)对缺血性结肠炎大鼠进行治疗,评价其治疗效果及探讨治疗机制,为临床用药提供理论依据。 三、使用不同剂量的阿魏酸钠(SF)对缺血性结肠炎大鼠进行治疗,评价SF治疗效果及探讨治疗机制,为治疗缺血性结肠炎提供新的途径。 方法:一、光化学法制备大鼠缺血性结肠炎模型:静脉注射光敏剂PSD-007后,用激光照射近端结肠。分别在照射后0、1、3、5、7、14天等不同时间点处死大鼠。检测结肠粘膜组织的LI、SOD、MDA和血清TNF-α的动态变化。 二、用不同剂量Lipo-PGEl治疗缺血性结肠炎大鼠:对照组(生理盐水)、小剂量组(0.5ug/kg)、中剂量组(1ug/kg)、大剂量组(2ug/kg)。各组均连续用药五天后全部处死,检测LI、SOD、MDA和6-keto-PGFl_α、TXB2及6-keto-PGFl_α/TXB2的变化,分析TNF-α、ICAM-1在结肠组织的表达情况。 三、用不同剂量的SF来治疗缺血性结肠炎大鼠,分为:对照组(生理盐水)、小剂量组(20mg/kg),中剂量组(40mg/kg)、大剂量组(80mg/kg),各组均连续用药五天后全部处死,检测LI、ET-1、SOD、MDA和6-keto-PGFl_α、TXB2及6-keto-PGFl_α/TXB2的变化,分析TNF-α、ICAM-1在结肠组织的表达情况。 结果:一、模型组大鼠经激光照射后出现进食水减少、活动度减弱、排便增多及暗红色血便等表现,LI随时间增加而加重,第5天达到最高点(3.25±0.46,P0.01),之后逐步下降,到第14天基本恢复正常;SOD活性随时间而下降,第3天到达最低点(19.12±6.70,P0.01),MDA含量随时间而增加,第3天到达最高点(30.38±4.41,P0.01);TNF-α含量随时间而增加,第5天到达最高点(1.89±0.38,P0.01)。上述指标均在第14天恢复正常。SOD与LI呈负相关,相关系数-0.7223(P0.01);MDA、TNF-α与LI呈正相关,
[Abstract]:Objective: 1. To establish a rat model of ischemic colitis and to explore its pathogenesis. Second, different doses of lipid microsphere prostaglandin E1 (Lipo-PGE1) were used to treat ischemic colitis rats, to evaluate the therapeutic effect and explore the therapeutic mechanism, to provide a theoretical basis for clinical use of drugs. Third, different doses of sodium ferulate (SF) were used to treat ischemic colitis rats, to evaluate the therapeutic effect of SF and explore the therapeutic mechanism, so as to provide a new way for the treatment of ischemic colitis. Methods: 1. The model of ischemic colitis in rats was made by photochemical method. After intravenous injection of photosensitizer PSD-007, the proximal colon was irradiated by laser. Rats were killed at 0, 1, 3, 5, 7, 14 days after irradiation. The dynamic changes of LI,SOD,MDA and serum TNF- 伪 in colonic mucosa were detected. Two, rats with ischemic colitis were treated with different doses of Lipo-PGEl: control group (saline), low dose group (0.5ug/kg), middle dose group (1ug/kg), high dose group (2ug/kg). All the rats in each group were killed for 5 days. The changes of LI,SOD,MDA, 6-keto-PGFL _ 伪, TXB2 and 6-keto-PGFL _ 伪 / TXB2 were detected, and the expression of TNF- 伪 and ICAM-1 in colon tissue were analyzed. The rats with ischemic colitis were divided into three groups: control group (saline), low dose group (20mg/kg), middle dose group (40mg/kg) and high dose group (80mg/kg). All the rats in each group were killed for 5 days. The changes of LI,ET-1,SOD,MDA, 6-keto-PGFL _ 伪, TXB2 and 6-keto-PGFL _ 伪 / TXB2 were detected, and the expression of TNF- 伪 and ICAM-1 in colon tissue were analyzed. Results: 1. After laser irradiation, the rats in the model group showed decreased intake of water, decreased activity, increased defecation and dark red blood stool. LI increased with the increase of time and reached the highest point on the 5th day (3.25 卤0.46, P0.01). After that, it gradually decreased and returned to normal on the 14th day. The activity of SOD decreased with time and reached the lowest point on the third day (19.12 卤6.70, P0.01). The content of), MDA increased with time and reached the highest point on the third day (30.38 卤4.41, P0.01). The content of TNF- 伪 increased with time and reached the highest point on the 5th day (1.89 卤0.38, P0.01). Sod was negatively correlated with LI, the correlation coefficient was-0.7223 (P0.01), and MDA,TNF- 伪 was positively correlated with LI.
【学位授予单位】：中国人民解放军军医进修学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R574.62;R-332
本文编号：2463117
[Abstract]:Objective: 1. To establish a rat model of ischemic colitis and to explore its pathogenesis. Second, different doses of lipid microsphere prostaglandin E1 (Lipo-PGE1) were used to treat ischemic colitis rats, to evaluate the therapeutic effect and explore the therapeutic mechanism, to provide a theoretical basis for clinical use of drugs. Third, different doses of sodium ferulate (SF) were used to treat ischemic colitis rats, to evaluate the therapeutic effect of SF and explore the therapeutic mechanism, so as to provide a new way for the treatment of ischemic colitis. Methods: 1. The model of ischemic colitis in rats was made by photochemical method. After intravenous injection of photosensitizer PSD-007, the proximal colon was irradiated by laser. Rats were killed at 0, 1, 3, 5, 7, 14 days after irradiation. The dynamic changes of LI,SOD,MDA and serum TNF- 伪 in colonic mucosa were detected. Two, rats with ischemic colitis were treated with different doses of Lipo-PGEl: control group (saline), low dose group (0.5ug/kg), middle dose group (1ug/kg), high dose group (2ug/kg). All the rats in each group were killed for 5 days. The changes of LI,SOD,MDA, 6-keto-PGFL _ 伪, TXB2 and 6-keto-PGFL _ 伪 / TXB2 were detected, and the expression of TNF- 伪 and ICAM-1 in colon tissue were analyzed. The rats with ischemic colitis were divided into three groups: control group (saline), low dose group (20mg/kg), middle dose group (40mg/kg) and high dose group (80mg/kg). All the rats in each group were killed for 5 days. The changes of LI,ET-1,SOD,MDA, 6-keto-PGFL _ 伪, TXB2 and 6-keto-PGFL _ 伪 / TXB2 were detected, and the expression of TNF- 伪 and ICAM-1 in colon tissue were analyzed. Results: 1. After laser irradiation, the rats in the model group showed decreased intake of water, decreased activity, increased defecation and dark red blood stool. LI increased with the increase of time and reached the highest point on the 5th day (3.25 卤0.46, P0.01). After that, it gradually decreased and returned to normal on the 14th day. The activity of SOD decreased with time and reached the lowest point on the third day (19.12 卤6.70, P0.01). The content of), MDA increased with time and reached the highest point on the third day (30.38 卤4.41, P0.01). The content of TNF- 伪 increased with time and reached the highest point on the 5th day (1.89 卤0.38, P0.01). Sod was negatively correlated with LI, the correlation coefficient was-0.7223 (P0.01), and MDA,TNF- 伪 was positively correlated with LI.
【学位授予单位】：中国人民解放军军医进修学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R574.62;R-332
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