冰温保存肾表皮细胞及其渗透特性的实验研究

发布时间：2019-05-05 09:23

【摘要】：以肾表皮细胞为研究对象,利用显微镜图像分析方法,研究了其在不同温度、不同渗透压下的渗透特性,其中包括细胞的非渗透体积、细胞膜对水和低温保护剂的渗透系数等。结果表明,在等渗溶液中,细胞的平均等渗体积Viso=2472.58μm3;细胞体积随溶液渗透压的变化规律符合Boyle van’t Hoff关系式,利用最小二乘法对实验数据进行线性拟合处理,得到非渗透体积Vb=0.22Viso;观察细胞在高渗低温保护剂溶液中的渗透响应,利用两参数模型,得到了不同温度下在二甲基亚砜、乙二醇、甘油溶液中肾表皮细胞对水的渗透系数(Lp)、对低温保护剂的渗透系数(Ps)及活化能(Ea);通过对细胞在冰温和常温下进行保存,测量细胞的存活率,得到了针对肾表皮细胞的最佳保存温度。基于上述结果,说明冰温保存细胞的方法在低温保存领域具有良好的应用前景。
[Abstract]:By means of the method of microscope image analysis, the osmotic characteristics of the cell in different temperature and osmotic pressure were studied with the method of microscope image analysis, including the non-osmotic volume of the cells, the permeability coefficient of the cell membrane to the water and the low-temperature protective agent, etc. The results showed that, in the isotonic solution, the average isoosmotic volume of the cells was 2472.58. m 3, the change of the cell volume with the osmotic pressure of the solution was in accordance with the Boyle van't Hoff relationship, and the experimental data were subjected to a linear fit treatment using the least square method to obtain the non-permeate volume of Vb = 0.22 Viso; The osmotic response of the cells in the solution of the hypertonic low-temperature protective agent was observed, and the permeability coefficient (Lp) of the water in the kidney-epidermal cells in the solution of dimethylethylene, ethylene glycol and glycerol at different temperatures was obtained by using the two-parameter model. The permeability coefficient (Ps) and the activation energy (Ea) of the low-temperature protective agent are measured, the survival rate of the cells is measured by storing the cells at the ice temperature and the normal temperature, and the optimal storage temperature for the renal epidermal cells is obtained. Based on the above results, the method for storing cells in the ice temperature has good application prospect in the field of low-temperature storage.
【作者单位】：天津商业大学天津市制冷技术重点实验室;天津大学中低温热能高效利用教育部重点实验室;
【基金】：国家自然科学基金项目(51076117)
【分类号】：R329.2

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