福建省结核分枝杆菌异烟肼耐药相关基因突变特征初步分析

发布时间：2019-05-15 01:29

【摘要】：目的了解福建省结核分枝杆菌异烟肼耐药相关基因的突变特征,为异烟肼耐药快速检测方法的建立提供一定的科学依据。方法对来源于福建省结核病耐药性监测30个监测点纳入的75株耐多药和10株全敏感结核分枝杆菌分离株,进行katG、inhA、oxyR-ahpC基因片段PCR扩增并测序分析,用RD105缺失基因检测法进行北京家族基因型鉴定,使用卡方检验分析相关性。结果 10株全敏感株未检测到突变。75株耐多药结核分枝杆菌检测到72株katG、inhA、oxyR-ahpC发生单一或联合基因突变,突变率为96.0%(72/75)。其中,65株(86.7%,65/75)发生katG突变,涉及5个位点,最常见位点突变的密码子是315,突变率为82.7%(62/75),最常见突变形式为Ser315Thr(77.3%,58/75);8株(10.7%,8/75)发生inhA突变,突变形式均为C(-15)T;5株(6.7%,5/75)发生oxyR-ahpC突变,突变形式为C(-39)T或C(-46)A。katG、inhA和oxyR-ahpC在北京家族基因型菌株和非北京家族基因型菌株中的突变率分别为83.9%(47/56)、12.5%(7/56)、7.1%(4/56)和94.7%(18/19)、5.3%(1/19)、5.3%(1/19),差异无统计学意义(P值分别为0.23、0.38、0.78)。结论福建省结核分枝杆菌异烟肼耐药性相关基因突变绝大多数发生在katG、inhA和oxyR-ahpC基因位点,且以katG突变为主。初步分析显示北京家族基因型菌株流行与异烟肼耐药基因突变特征无关。
[Abstract]:Objective to investigate the mutation characteristics of isoniazid resistance related genes in Mycobacterium tuberculosis in Fujian Province, and to provide some scientific basis for the establishment of rapid detection method for isoniazid resistance. Methods 75 strains of multi-drug resistance and 10 strains of fully sensitive Mycobacterium tuberculosis from 30 surveillance sites of tuberculosis resistance in Fujian Province were amplified by PCR and sequenced. The Beijing family genotyping was identified by RD105 deletion gene detection, and the correlation was analyzed by chi-square test. Results No mutation was detected in 10 fully sensitive strains. 72 strains of katG,inhA,oxyR-ahpC were detected to have single or combined gene mutation, the mutation rate was 96.0% (72 鈮，

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