MHCⅠ类分子对小鼠移植瘤成瘤影响的研究
发布时间：2019-06-18 08:28
【摘要】：目的:研究主要组织相容性复合体(Major histocompatibility complex,MHC)Ⅰ类分子不同的肿瘤细胞在与其MHC分子匹配/非匹配小鼠中的移植瘤成瘤情况,探索MHC分子在肿瘤发生中的作用。方法:1×104、3×104、5×104个小鼠结肠癌细胞CT26(H2d)分别接种BALB/c(H2d)和C57BL/6(H2b)小鼠,每个剂量对应设置皮下组和静脉组,对C57BL/6小鼠增加5×106、8×106和1×107数量组别;同样将1×105、5×105、1×106个小鼠黑色素瘤细胞B16F10(H2b)分别经皮下或静脉接种到两种小鼠体内,以相同接种量和接种方式下的不同小鼠间形成对比,14 d后观察各组成瘤情况。结果:在BALB/c小鼠成瘤实验中,CT26细胞数为1×104时静脉和皮下组均不成瘤,3×104时静脉组部分成瘤,皮下组不成瘤;5×104时两个组全部成瘤;注射1×105B16F10细胞时两组均不成瘤,5×105时静脉组全部成瘤,皮下组部分成瘤,1×106时两组全部成瘤。在C57BL/6小鼠实验中,皮下注射CT26细胞数5×106时不成瘤,6×106和8×106部分成瘤,1×107时皮下组全部成瘤,静脉组中CT26细胞达5×106也无肿瘤形成,大于此数量细胞注射时小鼠会因为肺栓塞死亡;对B16F10细胞,1×105时静脉和皮下组即可全部成瘤。结论:当肿瘤细胞与小鼠MHCⅠ表型相匹配,移植瘤成瘤所需细胞数远小于MHCⅠ表型不相匹配所需细胞数,说明MHC分子对肿瘤发生存在影响,此种差异为研究MHC与肿瘤的关系提供了线索,同时也提供了CT26、B16F10细胞株成瘤实验的精确数据。
[Abstract]:Aim: to study the tumorigenesis of tumor cells with different class I molecules of (Major histocompatibility complex,MHC) in mice matched with their MHC molecules, and to explore the role of MHC molecules in tumorigenesis. Methods: 1 脳 10 ~ 4, 3 脳 10 ~ 4, 5 脳 10 ~ 4 mice colon cancer cell line CT26 (H _ 2d) were inoculated with BALB/c (H _ 2d) and C57BL/6 (H _ 2B) mice, respectively. the subcutaneous group and vein group were set up for each dose, and the C57BL/6 mice were increased by 5 脳 10 ~ 6, 8 脳 10 ~ 6 and 1 脳 10 ~ 7 groups, respectively. At the same time, 1 脳 10 ~ 5, 5 脳 10 ~ 5 and 1 脳 10 ~ 6 mouse melanoma cells B16F10 (H _ 2b) were inoculated subcutaneously or intravenously into two kinds of mice, and the mice were compared with each other under the same inoculum size and vaccination mode. The tumor formation was observed 14 days later. Results: in the tumorigenesis experiment of BALB/c mice, there was no tumor in vein and subcutaneous group when the number of CT26 cells was 1 脳 10 ~ 4, partial tumorigenesis in vein group at 3 脳 10 ~ 4, no tumor in subcutaneous group at 5 脳 10 ~ 4, no tumor in both groups at 5 脳 10 ~ 4, no tumor in 5 脳 10 ~ 5 vein group, partial tumorigenesis in subcutaneous group and tumor formation in 1 脳 10 ~ 6 group. In the experiment of C57BL/6 mice, the number of CT26 cells injected subcutaneously was 5 脳 106, 6 脳 106 and 8 脳 106 were partial tumorigenesis, all tumors were formed in subcutaneous group at 1 脳 107, and there was no tumor formation in venous group when CT26 cells reached 5 脳 106, mice would die of pulmonary embolism when larger than this number of cells injection, and all of B16F10 cells could be formed in 1 脳 10 5 vein and subcutaneous group. Conclusion: when the tumor cells match the MHC 鈪，
本文编号：2501325
[Abstract]:Aim: to study the tumorigenesis of tumor cells with different class I molecules of (Major histocompatibility complex,MHC) in mice matched with their MHC molecules, and to explore the role of MHC molecules in tumorigenesis. Methods: 1 脳 10 ~ 4, 3 脳 10 ~ 4, 5 脳 10 ~ 4 mice colon cancer cell line CT26 (H _ 2d) were inoculated with BALB/c (H _ 2d) and C57BL/6 (H _ 2B) mice, respectively. the subcutaneous group and vein group were set up for each dose, and the C57BL/6 mice were increased by 5 脳 10 ~ 6, 8 脳 10 ~ 6 and 1 脳 10 ~ 7 groups, respectively. At the same time, 1 脳 10 ~ 5, 5 脳 10 ~ 5 and 1 脳 10 ~ 6 mouse melanoma cells B16F10 (H _ 2b) were inoculated subcutaneously or intravenously into two kinds of mice, and the mice were compared with each other under the same inoculum size and vaccination mode. The tumor formation was observed 14 days later. Results: in the tumorigenesis experiment of BALB/c mice, there was no tumor in vein and subcutaneous group when the number of CT26 cells was 1 脳 10 ~ 4, partial tumorigenesis in vein group at 3 脳 10 ~ 4, no tumor in subcutaneous group at 5 脳 10 ~ 4, no tumor in both groups at 5 脳 10 ~ 4, no tumor in 5 脳 10 ~ 5 vein group, partial tumorigenesis in subcutaneous group and tumor formation in 1 脳 10 ~ 6 group. In the experiment of C57BL/6 mice, the number of CT26 cells injected subcutaneously was 5 脳 106, 6 脳 106 and 8 脳 106 were partial tumorigenesis, all tumors were formed in subcutaneous group at 1 脳 107, and there was no tumor formation in venous group when CT26 cells reached 5 脳 106, mice would die of pulmonary embolism when larger than this number of cells injection, and all of B16F10 cells could be formed in 1 脳 10 5 vein and subcutaneous group. Conclusion: when the tumor cells match the MHC 鈪，
本文编号：2501325
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