用人骨髓间充质干细胞诱导抗砷细胞

发布时间：2019-07-02 19:45

【摘要】：研究目的:用低剂量NaAsO_2长期诱导人骨髓间充质干细胞(mesenchymal stem cells,MSCs),获得抗砷细胞,探讨机体抗砷机制。 研究方法:通过比较不同年龄人骨髓MSCs细胞倍增时间,细胞贴壁率等生物学性状及细胞的砷耐受性,筛选抗砷种子细胞;选取胎儿骨髓MSCs,采用细胞抑制率在5%-10~时的NaAsO_2浓度(1μmol/L)为砷诱导浓度,同时设一组不加砷培养的胎儿骨髓MSCs作为同步对照,用噻唑兰法(MTT)监测细胞抗砷性是否产生;采用氢化物发生—原子荧光方法检测细胞砷含量,二硫代二硝基苯甲酸直接显色法,比较抗砷细胞与同步对照组胞内GSH,GST等砷代谢指标的变化,验证细胞是否获得抗砷性,并初步探讨了细胞产生抗砷能力的机制。 结果:不同年龄人骨髓MSCs在细胞形态、生长特性等方面是相似的,胎儿骨髓MSCs的扩增潜能明显强于成人和老人骨髓MSCs。选择胎儿骨髓MSCs作为抗砷种子细胞,经过18周NaAsO_2诱导后,细胞对急性砷中毒的耐受性明显提高,排砷能力增强,细胞内GSH和GST活性增高。 结论:胎儿骨髓MSCs体外增殖分裂能力最强。人骨髓MSCs在低剂量NaAsO_2长期诱导下,能分化为具有抗砷能力的细胞。
[Abstract]:Objective: to obtain anti-arsenic cells from human bone marrow mesenchymal stem cells (mesenchymal stem cells,MSCs) induced by low dose NaAsO_2 for a long time, and to explore the mechanism of arsenic resistance. Methods: the anti-arsenic seed cells were screened by comparing the biological characteristics such as doubling time, cell adhesion rate and arsenic tolerance of human bone marrow MSCs cells of different ages. Fetal bone marrow MSCs, was treated with NaAsO_2 concentration (1 渭 mol / L) when the cell inhibition rate was 5% 鈮，

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