人免疫缺陷病毒重组非复制型痘苗病毒的构建及其免疫原性研究
发布时间：2019-07-09 20:02
【摘要】： HIV是人类的顽强对手,自从1981年发现艾滋病二十多年过去了,HIV疫苗研究虽然取得了重要进展(一些候选疫苗在动物实验中可部分阻断HIV感染和延缓AIDS发生),但仍然没有成功,HIV疫苗的研究对当今科学提出了前所未有的挑战。HIV具有高度变异性、潜伏感染性和免疫细胞靶向性,HIV引起的是慢性感染,在T细胞、巨噬细胞和单核细胞中建立病毒贮存库,在这些细胞中部分病毒发生整合,以静止的前病毒形式存在;病毒具有高度变异性,强大的免疫逃逸能力;免疫细胞本身就是病毒攻击的靶细胞,由于存在诱导感染增强抗体的危险性,以及缺乏理想的感染动物模型等,这些都给AIDS疫苗的研制带来了很大的困难。 本研究以中国HIV-1流行株保守区域Gag-Pol序列为目的基因,在密码子优化的基础上,构建了用于同源重组的穿梭质粒PSC-11-GagPol,经同源重组及筛选检测,得到特异性表达HIV-1 Gag-Pol蛋白的重组痘苗病毒rMVA-Psc11-GagPol,经过体液及细胞免疫结果的分析,对rMVA-Psc11-GagPol的免疫原性进行了研究。
文内图片：
图片说明：HIV感染T细胞示意图
[Abstract]:HIV is a tenacious opponent of human beings. More than 20 years after the discovery of AIDS in 1981, although some candidate vaccines can partially block HIV infection and delay the occurrence of AIDS in animal experiments, it is still not successful. HIV vaccine research poses an unprecedented challenge to today's science. HIV has a high degree of variability, latent infection and immune cell targeting. HIV causes chronic infection. Virus storage is established in T cells, macrophages and monocytes. Some of the viruses in these cells are integrated and exist in the form of static proviruses. The virus has a high degree of variability and strong immune escape ability, and the immune cells themselves are the target cells attacked by the virus. Due to the risk of inducing infection and enhancing antibodies, as well as the lack of an ideal animal model of infection, these bring great difficulties to the development of AIDS vaccine. In this study, the conserved region Gag-Pol sequence of Chinese HIV-1 epidemic strain was used as the target gene, and on the basis of codon optimization, the shuttle plasmid PSC-11-GagPol, for homologous recombination was constructed. The recombinant vaccinia virus rMVA-Psc11-GagPol, which specifically expressed HIV-1 Gag-Pol protein, was obtained by humoral and cellular immunity, and the immunogenicity of rMVA-Psc11-GagPol was studied.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392
本文编号：2512410
文内图片：
图片说明：HIV感染T细胞示意图
[Abstract]:HIV is a tenacious opponent of human beings. More than 20 years after the discovery of AIDS in 1981, although some candidate vaccines can partially block HIV infection and delay the occurrence of AIDS in animal experiments, it is still not successful. HIV vaccine research poses an unprecedented challenge to today's science. HIV has a high degree of variability, latent infection and immune cell targeting. HIV causes chronic infection. Virus storage is established in T cells, macrophages and monocytes. Some of the viruses in these cells are integrated and exist in the form of static proviruses. The virus has a high degree of variability and strong immune escape ability, and the immune cells themselves are the target cells attacked by the virus. Due to the risk of inducing infection and enhancing antibodies, as well as the lack of an ideal animal model of infection, these bring great difficulties to the development of AIDS vaccine. In this study, the conserved region Gag-Pol sequence of Chinese HIV-1 epidemic strain was used as the target gene, and on the basis of codon optimization, the shuttle plasmid PSC-11-GagPol, for homologous recombination was constructed. The recombinant vaccinia virus rMVA-Psc11-GagPol, which specifically expressed HIV-1 Gag-Pol protein, was obtained by humoral and cellular immunity, and the immunogenicity of rMVA-Psc11-GagPol was studied.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392
【参考文献】
相关期刊论文 前2条
1 胡国龄;艾滋病研究进展[J];实用预防医学;2000年03期
2 裘炯良,郑剑宁,赵玉婉;艾滋病研究进展[J];检验检疫科学;2003年01期
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