姬松茸多糖促进OVA抗原免疫反应的研究

发布时间：2019-08-06 11:26

【摘要】：目的:探讨姬松茸多糖(ABP-AW1)促进OVA抗原诱导Th1/Th2免疫反应的研究。方法:ICR小鼠随机分为PBS免疫组、OVA免疫组、DT免疫1、2、3组和Alum免疫组。第1次免疫后,间隔2周再免疫第2次。采集二免2周后小鼠血液,间接ELISA法测定Ig G及其亚类抗体水平,取重要脏器HE染色观察ABP-AW1佐剂对主要脏器继发的损伤反应。结果:OVA免疫组血清中抗OVA特异性抗体Ig G的产生与PBS免疫组、DT免疫组2、Alum免疫组比较差异均有统计学意义(P0.05);OVA免疫组血清中抗OVA特异性抗体Ig G2b的产生与PBS免疫组、DT免疫组2、DT免疫组3比较差异均有统计学意义(P0.05);OVA免疫组血清中抗OVA特异性抗体Ig G1的产生与其余各组比较差异均有统计学意义(P0.05)。未见主要脏器的损伤反应,无继发性的免疫损伤。结论:ABP-AW1佐剂可同时促进OVA免疫小鼠Th1和Th2型免疫反应,调节抗原刺激小鼠Th1/Th2失衡的作用,具有不同于铝佐剂的Th1优势反应。
[Abstract]:Objective: to investigate the effect of Agaricus blazei polysaccharide (ABP-AW1) on Th1/Th2 immune response induced by OVA antigen. Methods: ICR mice were randomly divided into PBS immunization group, OVA immunization group, DT immunization group 1, 2, 3 groups and Alum immunization group. After the first immunization, the second immunization was performed at intervals of 2 weeks. The blood of mice was collected after 2 weeks of immunization, and the levels of Ig G and its subclass antibodies were measured by indirect ELISA method. The HE staining of important organs was taken to observe the secondary injury response of ABP-AW1 adjuvants to the main organs. Results: the production of anti-OVA specific antibody Ig G in OVA immunized group was significantly different from that in PBS immunized group and DT immunized group (P 0.05). The production of anti-OVA specific antibody Ig G2b in); OVA immunized group was significantly different from that in PBS immunized group and DT immunized group 3 (P 0.05). The production of anti-OVA specific antibody Ig G 1 in OVA immunized group was significantly different from that in other groups (P 0.05). There was no injury reaction of the main organs and no secondary immune injury. Conclusion: ABP-AW1 adjuvant can promote the immune response of Th1 and Th2 type in mice immunized with OVA at the same time, regulate the imbalance of Th1/Th2 stimulated by antigen in mice, and has the dominant response of Th1 which is different from that of aluminum adjuvant.
【作者单位】：齐齐哈尔医学院第一附属医院;齐齐哈尔医学院;
【基金】：黑龙江省教育厅面上项目(12531800)
【分类号】：R392

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