Protective effects of terminal ileostomy against bacterial t

发布时间：2020-12-24 00:49

　　AIM:To investigate the effects of terminal ileostomy on bacterial translocation（BT）and systemic inflammation after intestinal ischemia/reperfusion（I/R）injury in rats.METHODS:Thirty-two rats were assigned to either the sham-operated group,I/R group,I/R+resection and anastomosis group,or the I/R+ileostomy group.The superior mesenteric artery was occluded for 60 min.After 4 h,tissue samples were collected for analysis.BT was assessed by bacteriologic cultures,intestinal permeability and serum level... 

【文章来源】：World Journal of Gastroenterology. 2014年47期

【文章页数】：9 页

【文章目录】：
INTRODUCTION
MATERIALS AND METHODS
    Animals
    Operative procedure
    Microbiologic analysis
    Wet-to-dry weight ratio
    Assay of intestinal permeability
    Endotoxin and serum levels of TNF-α, IL-6 and IL-10assays
    Assay of myeloperoxidase activity
    Histopathology
    Statistical analysis
RESULTS
    Bacterial translocation
    Ileal bacterial counts
    W/D weight ratio
    Assay of intestinal permeability
    Endotoxin assays
    Assay of TNF-α, IL-6 and IL-10 in serum
    Assay of MPO activity
    Histopathology
DISCUSSION
COMMENTS



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