转录抑制LPL表达的ZHX2蛋白功能域的鉴定
发布时间：2022-01-05 17:25
ZHX2（zinc fingers and homoboxes 2）,即锌指结构与同源结构域蛋白2,是一个转录抑制因子,与ZHX1、ZHX3组成锌指结构及同源结构域家族蛋白。研究发现,ZHX2在肝癌、肺癌、霍奇金淋巴瘤等多种恶性肿瘤疾病中表达下调,因此ZHX2被鉴定为一个新的抑癌基因。此外,ZHX2尚可转录抑制肝癌细胞中重要的肿瘤标记物甲胎蛋白（AFP）、GPC3的表达,并通过调控细胞周期蛋白,抑制细胞的过度增殖,进一步说明ZHX2在抑制肿瘤发生和进程中起着重要作用,并为肝癌的诊断和预后监测提供了新的候选分子。与其他遗传背景相似的小鼠相比,Balb/cJ小鼠肝脏内的ZHX2表达水平显著降低,并可以避免高脂饮食饲喂诱导的高脂血症。通过数量性状位点图谱鉴定发现,脂质代谢相关基因LPL的表达水平与ZHX2表达水平呈现相反的表达趋势,这也许是因为饲喂高脂饮食后,Balb/cJ小鼠肝脏中高表达的LPL能摄取更多血浆甘油三酯,使血浆中甘油三酯的水平低于其他品系的小鼠。LPL全称脂蛋白脂肪酶,是机体分解甘油三酯的关键酶。LPL通过肝素结合位点以同源二聚体的形式锚定在血管内皮细胞表面,负责摄取载脂蛋白...
【文章来源】：山东大学山东省 211工程院校 985工程院校 教育部直属院校
【文章页数】：72 页
【学位级别】：硕士
【部分图文】：
图２．肝癌细胞系中ＺＨＸ２?（２４２－４４６?ａａ）是抑制ＬＰＬ表达的较小功能区??Ｆｉｇｕｒｅ２?ＺＨＸ２?（２４２－４４６ａａ）?ｉｓ?ｔｈｅ?ｆｕｎｃｔｉｏｎ?ｄｏｍａｉｎ?ｆｏｒ??ｉｎｈｉｂｉｔｉｎｇ?ＬＰＬ?ｅｘｐｒｅ巧ｉｏｎ?ｉｎ?ｈｅｐａｔｏｍａ?ｃｅｌｌ?ｌｉｎｅｓ??．－
图３．?ＺＨＸ２?（２４２－４４６ａａ）抑制Ｌ化核也启动子活性??Ｆｉｇｕｒｅ?３?ＺＨＸ２?（２４２－４４６ａａ）?ｉｎｈｉｂｉｔｓ?ＬＰＬ?ｃｏｒｅ?ｐｒｏｍｏｔｅｒ?ａｃｔｉｖｉｔｙ??
图４．ＣｏｎＡ诱导肝炎模型后肝脏ＺＨＸ２表达上调??Ｆｉｇｕｒｅ４?之ＨＸ２?ｉｓ?ｕｐ－ｒｅｇｕｌａｔｅｄ?ｉｎ?化ｅ?ｍｏｕｓｅ?ｌｉｖｅｒ?ｏｆ?ＣｏｎＡ?ｉｎｄｕｃｅｄ?ｈｅｐａｔｉｔｉｓ??１．?２?ＬＰＳ／Ｇａ?ＩＮ肝炎模型肝组织中的ＺＨＸ２表达上巧??
【参考文献】：
期刊论文
[1]Immune mechanisms of Concanavalin A model of autoimmune hepatitis[J]. Hai-Xia Wang,College of Sciences,Anhui Science and Technology University,Fengyang 233100,Anhui Province,China Man Liu,Shun-Yan Weng,Jing-Jing Li,Chao Xie,Hong-Lin He,Wen Guan,Yun-Sheng Yuan,Shanghai Municipality Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China Jin Gao,Laboratory of Regeneration,School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China. World Journal of Gastroenterology. 2012(02)
[2]Kupffer cells contribute to concanavalin A-induced hepatic injury through a Th1 but not Th17 type response-dependent pathway in mice[J]. Lin Chen,Xiao-Jun Xie,Yu-Fu Ye,Lin Zhou,Hai-Yang Xie,Qin-Fen Xie, Jiong Tian and Shu-Sen ZhengZhejiang University School of Medicine,Hangzhou 310003,ChinaKey Laboratory of Combined Multi-organ Transplantation,Ministry of Public Health;Department of Hepatobiliary and Pancreatic Surgery Department of Nephrology,First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China. Hepatobiliary & Pancreatic Diseases International. 2011(02)
本文编号：3570763
【文章来源】：山东大学山东省 211工程院校 985工程院校 教育部直属院校
【文章页数】：72 页
【学位级别】：硕士
【部分图文】：
图２．肝癌细胞系中ＺＨＸ２?（２４２－４４６?ａａ）是抑制ＬＰＬ表达的较小功能区??Ｆｉｇｕｒｅ２?ＺＨＸ２?（２４２－４４６ａａ）?ｉｓ?ｔｈｅ?ｆｕｎｃｔｉｏｎ?ｄｏｍａｉｎ?ｆｏｒ??ｉｎｈｉｂｉｔｉｎｇ?ＬＰＬ?ｅｘｐｒｅ巧ｉｏｎ?ｉｎ?ｈｅｐａｔｏｍａ?ｃｅｌｌ?ｌｉｎｅｓ??．－
图３．?ＺＨＸ２?（２４２－４４６ａａ）抑制Ｌ化核也启动子活性??Ｆｉｇｕｒｅ?３?ＺＨＸ２?（２４２－４４６ａａ）?ｉｎｈｉｂｉｔｓ?ＬＰＬ?ｃｏｒｅ?ｐｒｏｍｏｔｅｒ?ａｃｔｉｖｉｔｙ??
图４．ＣｏｎＡ诱导肝炎模型后肝脏ＺＨＸ２表达上调??Ｆｉｇｕｒｅ４?之ＨＸ２?ｉｓ?ｕｐ－ｒｅｇｕｌａｔｅｄ?ｉｎ?化ｅ?ｍｏｕｓｅ?ｌｉｖｅｒ?ｏｆ?ＣｏｎＡ?ｉｎｄｕｃｅｄ?ｈｅｐａｔｉｔｉｓ??１．?２?ＬＰＳ／Ｇａ?ＩＮ肝炎模型肝组织中的ＺＨＸ２表达上巧??
【参考文献】：
期刊论文
[1]Immune mechanisms of Concanavalin A model of autoimmune hepatitis[J]. Hai-Xia Wang,College of Sciences,Anhui Science and Technology University,Fengyang 233100,Anhui Province,China Man Liu,Shun-Yan Weng,Jing-Jing Li,Chao Xie,Hong-Lin He,Wen Guan,Yun-Sheng Yuan,Shanghai Municipality Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China Jin Gao,Laboratory of Regeneration,School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China. World Journal of Gastroenterology. 2012(02)
[2]Kupffer cells contribute to concanavalin A-induced hepatic injury through a Th1 but not Th17 type response-dependent pathway in mice[J]. Lin Chen,Xiao-Jun Xie,Yu-Fu Ye,Lin Zhou,Hai-Yang Xie,Qin-Fen Xie, Jiong Tian and Shu-Sen ZhengZhejiang University School of Medicine,Hangzhou 310003,ChinaKey Laboratory of Combined Multi-organ Transplantation,Ministry of Public Health;Department of Hepatobiliary and Pancreatic Surgery Department of Nephrology,First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China. Hepatobiliary & Pancreatic Diseases International. 2011(02)
本文编号：3570763
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