qPCR检测结核杆菌在肺和胸膜上皮样肉芽肿性疾病诊断中的意义

发布时间：2018-07-09 11:44

本文选题：结核 + 分枝杆菌　；参考：《临床与实验病理学杂志》2017年10期

【摘要】：目的探讨荧光定量聚合酶链反应(Real-time polymerase chain reaction,q PCR)法在诊断及鉴别诊断肺和胸膜结核病和其他肉芽肿性疾病中的意义。方法收集经病理学检查发现上皮样肉芽肿性病变的肺和胸膜活检的石蜡包埋组织142例,应用q PCR法检测结核分枝杆菌DNA,同时行抗酸染色,并与临床资料对比进行回顾性分析。结果在142例肺胸膜活检标本中,60例临床诊断确诊为结核病。采用q PCR法检测结核分枝杆菌DNA阳性者58例,敏感性为96.67%,特异性为100%;抗酸染色阳性者17例,敏感性为28.33%,特异性为97.56%,差异有统计学意义(χ~2=21.47,P0.001)。结论结核分枝杆菌核酸检测有助于在石蜡包埋的肺和胸膜小活检组织中快速、准确的诊断结核病,可用于疑似结核的肉芽肿性病变的鉴别诊断。
[Abstract]:Objective to investigate the significance of real-time polymerase chain reactionqPCR in the diagnosis and differential diagnosis of pulmonary and pleural tuberculosis and other granulomatous diseases. Methods 142 cases of lung and pleural biopsy paraffin embedded tissues with epithelial granulomatous lesions were collected. The DNA of Mycobacterium tuberculosis was detected by qPCR and acid fast staining was performed, and the clinical data were analyzed retrospectively. Results 60 of 142 pulmonary pleural biopsy specimens were diagnosed as tuberculosis. The sensitivity was 96.677.The specificity was 100 and the sensitivity was 28.33 and the specificity was 97.56 (蠂 ~ 2 / 2) in 17 cases of positive acid-fast staining (蠂 ~ (2 +) 21.47 ~ (7) P 0.001) by using qPCR method to detect the DNA positive of Mycobacterium tuberculosis in 58 cases (蠂 ~ (2) 2 + 21.47) (P ~ (0.001). Conclusion Detection of Mycobacterium tuberculosis nucleic acid is helpful for rapid and accurate diagnosis of tuberculosis in paraffin-embedded lung and pleural small biopsy tissues, and can be used for differential diagnosis of granulomatous lesions suspected of tuberculosis.
【作者单位】：福建省立医院病理科/福建省立临床医学院;
【基金】：国家青年自然科学基金(81201794) 福建省卫生系统中青年骨干人才培养项目(2015-ZQN-ZD-3) 福建省自然科学基金(2012J01117) 福建省医学创新课题(2012-CX-3)
【分类号】：R52

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