天津地区KI和WU多瘤病毒在儿童急性呼吸道感染中的分子流行病学研究

发布时间：2018-01-30 00:50

本文关键词： KI多瘤病毒 WU多瘤病毒急性呼吸道感染基因同源性儿童　出处：《中国当代儿科杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的了解KI和WU多瘤病毒(KIPy V和WUPy V)与天津地区儿童急性呼吸道感染的关系。方法采用PCR扩增方法对2011年1月至2013年12月收集的3 730份来自天津地区的急性呼吸道感染患儿鼻咽分泌物标本进行KIPy V和WUPy V基因检测,同时所有标本均用直接免疫荧光法检测7种常见呼吸道病毒。选取部分KIPy V和WUPy V PCR阳性产物进行测序,利用测序序列与已知序列比对并绘制进化树。再从中选取两株KIPy V VP1基因克隆到T载体上,进行序列测定和分析,并将核苷酸序列提交Gen Bank。结果 3 730份标本中,KIPy V阳性检出453份(12.14%),WUPy V阳性检出63份(1.69%)。KIPy V平均感染率在6~7月明显偏高,WUPy V平均感染率在2~3月达到一个小高峰。两种多瘤病毒感染的阳性患儿年龄主要集中在3岁以内。KIPy V和WUPy V与其他7种呼吸道病毒存在混合感染,混合感染率为2.31%(86/3 730);WUPy V和KIPy V混合感染9份。选取的35份KIPy V阳性PCR产物序列与Gen Bank已公布的KIPy V序列的同源性在94%~100%之间;选取的12份WUPy V阳性PCR产物序列与Gen Bank已公布的WUPy V序列的同源性在95%~100%之间。两株KIPy V VP1基因序列已被Gen Bank收录,登录号为KY465925和KY465926。结论天津地区部分儿童的急性呼吸道感染可能与WUPy V和KIPy V感染相关。KIPy V感染多发于夏季,WUPy V感染多发于春季。KIPy V和WUPy V与国内外流行株间差异较小,基因组相对稳定。
[Abstract]:Objective to understand Ki and WU polytumour virus (KIPy V) and WUPy virus (WUPy V). The relationship between acute respiratory tract infection (ARI) in children and children in Tianjin area. Methods PCR amplification method was used to analyze the data collected from January 2011 to December 2013. KIPy V and WUPy V genes were detected in 730 nasopharyngeal secretions from children with acute respiratory infection in Tianjin. At the same time, seven common respiratory viruses were detected by direct immunofluorescence assay. Partial KIPy V and WUPy V PCR positive products were sequenced. Two KIPy V VP1 genes were selected to be cloned into T vector and sequenced and analyzed. The nucleotide sequence was submitted to Gen Bank.Results 453 KIPY V positive specimens were detected in 453 samples. The average infection rate of 63 WUPy V positive samples was significantly higher from 6 to July. The average infection rate of WUPy V reached a small peak from 2 to March. The age of the positive children with two kinds of polytumour virus infection was mainly within 3 years old. KIPY V and WUPy. V has mixed infection with other 7 respiratory viruses. The mixed infection rate was 2.31% and 3.730%; 9 WUPy V and KIPy V mixed infection. 35 KIPy V positive PCR product sequences and KIPy published by Gen Bank were selected. The homology of V sequence was between 94% and 100%. The homology between the 12 WUPy V positive PCR products and the published WUPy V sequence of Gen Bank was 95% and 100% respectively. VP1 gene sequence has been included in Gen Bank. The registration numbers are KY465925 and KY465926.Conclusion the acute respiratory infection in some children in Tianjin area may be related to WUPy V and KIPy V infection. V infection occurred in summer. WUPy V infection mainly occurred in spring. KIPY V and WUPy V had little difference with the epidemic strains at home and abroad, and the genome was relatively stable.
【作者单位】：天津市儿童医院/天津市儿科研究所;天津市儿童医院呼吸科;天津科技大学生物工程学院;
【基金】：国家自然科学基金项目（31370205）天津市卫生局科技基金（2011KZ33）
【分类号】：R725.6
【正文快照】： 在世界范围内,病毒感染是导致儿童呼吸道感染发病和死亡的重要原因之一[1]。目前为止,即使使用最先进的诊断技术,仍然只有50%的呼吸道病原可以被确定[2]。多瘤病毒属病毒含有双链DNA基因组,可以感染多种生物体,如禽类、啮齿动物和一些灵长类动物[3]。2007年之前,只有两种多瘤

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