七味白术散治疗小儿轮状病毒肠炎的肠道宏基因学相关研究
本文关键词: 七味白术散 轮状病毒肠炎 肠道菌群 宏基因组学 出处:《广州中医药大学》2014年硕士论文 论文类型:学位论文
【摘要】:目的: 利用宏基因组学方法,通过动物及临床实验,研究七味白术散治疗轮状病毒(RV)肠炎中对肠道宏基因组的影响,以利于探索肠道微生态在中药治疗轮状病毒肠炎中的可能作用。 方法: 本项研究包括动物实验及临床实验两阶段。在动物实验阶段中,选择5日龄昆明小鼠为研究对象,按窝别随机分为七味白术散组、模型对照组及健康对照组;七味白术散组及模型对照组均以轮状病毒(RV)悬液经口感染,造模成功后七味白术散组以七味白术散复方煎液经口灌胃,模型对照组及健康对照组则喂以等量的生理盐水;3日后采集各组乳鼠的结肠内容物标本,并应用宏基因组学方法,检测及对比其肠道菌群结构。在临床实验阶段中,选取1例辨证为脾虚证的12个月大的轮状病毒肠炎患儿,应用宏基因组学方法,检测及对比其服用七味白术散前后肠道菌群的变化。 结果: 动物和临床实验结果显示,RV感染后的乳鼠和婴儿之间,其肠道菌群结构差异很大,例如,在门的水平上,前者以变形菌(Proteobacteria)为主,而后者则以放线菌(Actinobacteria)为主。七味白术散治疗后的RV感染乳鼠与模型对照组、健康对照组之间,以及七味白术散治疗后的RV感染婴儿前后,其肠道菌群结构均存在显著不同。对选择的肠道关键菌群——双歧杆菌和乳杆菌,在动物实验中,七味白术散组的肠道双歧杆菌含量高于模型对照组,而乳杆菌含量则显著低于模型对照组;然而,对RV感染婴儿,其肠道双歧杆菌含量在七味白术散治疗后显著下降,而乳杆菌含量则在治疗后显著增高。 结论: 七味白术散的治疗改变了RV感染乳鼠和婴儿的肠道菌群结构,但很可能并不是通过影响单一特定菌群来起作用;另一方面,由于乳鼠和婴儿之间的肠道菌群结构差异很大,功能基因组学的分析可能更有价值。
[Abstract]:Objective: The effects of Qiwei Baizhu Powder on intestinal macrogenome in the treatment of rotavirus RV) enteritis were studied by means of macrogenomics and animal and clinical experiments. In order to explore the role of intestinal microecology in the treatment of rotavirus enteritis. Methods: This study includes two stages: animal experiment and clinical experiment. In the animal experiment stage, the 5-day-old Kunming mice were selected as the research objects, and randomly divided into Qiwei Baizhu powder group, model control group and healthy control group according to their nest; Qiwei Baizhu Powder group and model control group were infected by rotavirus RV) suspension. After the successful establishment of the model, Qiwei Baizhu Powder group was orally perfused with Qiwei Baizhu Powder compound decoction. The model control group and the healthy control group were fed with the same amount of normal saline. 3 the colonic contents of each group of neonatal rats were collected, and the intestinal microflora structure was detected and compared by means of macrogenomics. A case of rotavirus enteritis aged 12 months with spleen deficiency syndrome was selected to detect and compare the changes of intestinal flora before and after taking Qiwei Baizhu Powder by macrogenomics. Results: Animal and clinical trials have shown that the intestinal flora structure varies significantly between newborn mice and infants infected with RV, for example, at the portal level. The former is Proteobacteria (Proteobacteria). The latter was mainly actinobacteriae). After treatment with Qiwei Atractylodes powder, there were three groups: model control group, model control group and healthy control group. The intestinal flora structure of infants infected with RV after treatment with Qiwei Baizhu Powder was significantly different. The key intestinal flora bifidobacterium and Lactobacillus were selected in animal experiment. The intestinal bifidobacterium content in Qiwei Baizhu Powder group was higher than that in the model control group, while the Lactobacillus content was significantly lower than that in the model control group. However, for infants infected with RV, the intestinal bifidobacterium content decreased significantly after treatment with Qiwei Baizhu Powder, while the content of Lactobacillus increased significantly after treatment. Conclusion: The treatment of Qiwei Baizhu Powder changed the intestinal microflora structure of newborn mice and infants infected with RV, but probably not by affecting a single specific flora. On the other hand, the analysis of functional genomics may be more valuable because of the large differences in intestinal flora structure between newborn mice and infants.
【学位授予单位】:广州中医药大学
【学位级别】:硕士
【学位授予年份】:2014
【分类号】:R272
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