儿童结核病结核分枝杆菌临床分离株中esxA、fbpB、Rv2660c基因多态性研究

发布时间：2018-02-01 02:18

本文关键词： 结核分枝杆菌 esxA fbpB Rvc 基因多态性　出处：《第三军医大学学报》2017年17期 　论文类型：期刊论文

【摘要】：目的对抗原编码基因esxA、fbpB、Rv2660c进行测序,了解esxA、fbpB、Rv2660c的基因多态性。方法选取135株结核分枝杆菌(Mycobacterium tuberculosis,MTB)临床分离株,运用PCR方法扩增目的基因esx A、fbp B、Rv2660c并进行测序,与结核标准株H37Rv基因序列以及从免疫表位数据库(immune epitope database,IEDB)中查询到的表位序列进行对比,分析esx A、fbp B、Rv2660c基因序列中的变异特征。结果在135株菌株中,esx A、Rv2660c序列暂未发现基因变异。fbpB共66株(48.89%)发现了基因变异,5个单核苷酸多态性(single nucleotide polymorphism,SNP)位点共造成8个(14.81%)T细胞抗原表位以及9个(24.32%)B细胞抗原表位序列的多态性。fbpB基因的d N值为0.000 120,d S为0.002 126,其中T细胞表位区与非T细胞表位区的d N值分别为0.000 067和0.000 408,d S分别为0.002 382和0.000 470,B细胞表位区dN和dS值分别为0.000 063和0.002 380,非B细胞表位区dN和dS均为0。结论 esxA、Rv2660c基因序列较保守,fbp B存在基因多态性,预测会对蛋白Ag85B的免疫功能造成影响,从而影响H56的免疫有效性。
[Abstract]:Objective to sequence the antigen-coding gene esxAmfbpBnrv2660c and to understand the expression of esxAfbpB. Methods 135 clinical isolates of Mycobacterium tuberculosisus were selected. The target gene esx AfBP BV2660c was amplified by PCR and sequenced. The results were compared with the H37Rv gene sequence of TB standard strain and the epitope sequence obtained from immune epitope database (immune epitope database IEDB). The variation characteristics of esx AfBP rv2660c gene were analyzed. Gene mutation was not found in Rv2660c sequence. 66 strains of FBpB were found. Five single nucleotide polymorphisms (SNP) single nucleotide polymorphism. SNPs cause 8 T cell epitopes and 9 T cell epitopes. The polymorphism of B cell epitope sequence. The dN value of fbpB gene was 0.000120. D S was 0.002 126, and the N values of T cell epitope and non T cell epitope were 0.000067 and 0.000408, respectively. DS values were 0.002 382 and 0.000 470 B cell epitopes, respectively. The values of DN and DS were 0.000 063 and 0.002 380, respectively. The number of dN and DS in non-B cell epitope was 0.Conclusion the sequence of esxAcon-Rv2660c gene is more conserved than that of conserved FBP B gene. Prediction will affect the immune function of protein Ag85B, thus affecting the immune effectiveness of H56.
【作者单位】：重庆医科大学附属儿童医院感染科儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室;Department
【分类号】：R529.9
【正文快照】： 部重点实验室,儿童发育重大疾病国家国际科技合作基地,儿科学重庆市重点实验室1;2Department of Epidemiology,School of Public Health,University of Michigan,1415 Washington Heights,Ann Arbor,MI 48109-2029,USA)结核病(tuberculosis,TB)作为威胁全人类健康的慢性传染病

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