手足口病病毒EV71衣壳蛋白VP1基因特征分析

发布时间：2018-02-12 23:22

本文关键词： 手足口病 EV VP 同源性分析　出处：《中国病原生物学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的分析幼童手足口病病毒EV71衣壳蛋白VP1基因特征,为手足口病临床预防和控制提供指导。方法随机收集604例手足口病幼童患者标本,培养病毒株并分离鉴定。采用RT-PCR扩增EV71及其VP1区基因,经基因产物测序分析毒株的核苷酸和氨基酸序列同源性。结果604例手足口病幼童患者标本中,男性患者369例(61.09%)和女性235例(38.91%);(34.60%).1~岁179例(29.64%),2~岁216例(35.76%)和3~岁209例(34.60%)。共分离出病毒129株,分离率21.36%,其中EV71 61株(占47.29%),CoxA16 28株(占21.71%),其他肠道病毒40株(占31.01%)。61株EV71的VP1区基因序列间核苷酸同源性95.7%~100.0%,氨基酸同源性97.3%~100.0%。与C4a亚型代表株的核苷酸同源性96.4%~98.9%,氨基酸同源性99.0%~100.0%。与CoxA16原始株G-10、A、B基因型代表株VP1区以及C4b、C5亚型代表株的核苷酸同源性分别为66.0%~68.9%、78.6%~82.8%、83.2%~85.5%、95.2%~97.4%和84.5%~87.6%,氨基酸同源性分别为71.4%~75.7%、90.5%~93.6%、95.1%~96.0%、99.0%~99.8%和97.6%~99.4%。结论手足口病发病以3~4岁男性幼童为主,且以EV71为主。所分离的EV71流行株属于C4亚型。
[Abstract]:Objective to analyze the characteristics of EV71 capsid protein VP1 gene of hand, foot and mouth disease virus in young children, and to provide guidance for clinical prevention and control of hand foot and mouth disease. Methods 604 samples of young children with hand foot and mouth disease were collected randomly. EV71 and its VP1 region gene were amplified by RT-PCR. The nucleotide and amino acid sequence homology of the virus strain was analyzed by sequencing the gene products. Results in 604 samples of young children with HFMD, the nucleotide and amino acid sequence homology was analyzed. A total of 129 virus strains were isolated from 369 male patients (61.09%) and 235 women (38.91 cases) and 179 cases (29.6464 years old, 179 cases) and 209 cases (3 ~ 209 cases), respectively. The isolation rate was 21.366.There were 61 strains of EV71 (47.29k.CoxA1628), 40 strains of other enterovirus (31.01%), 95.7% 100.0 homology of VP1 region, 97.3% amino acid homology, 96.498.9 nucleotide homology with C4a subtype representative strain, 96.498.9 amino acid homology, and 98.9% amino acid homology with C4a subtype representative strain. The nucleotide homology was 66.0% in the VP1 region and 68.0% in the C4bC5 subtype, respectively, and 82.82.83.25.5in the position of 95.297.4% and 84.57.6%, the amino acid homology was 71.4% 75.70.5% and 93.695%, respectively. The amino acid homology was 99.099.099.8% and 97.66.40.Conclusion Hand-mouth disease is mainly developed in 34-year-old male young children, and 96.099.099.099.40.ConclusionThe amino acid homology is 71.4% and 84.57.66.The amino acid homology is 71.4%, 95.70.5% and 93.65.95%, respectively, and the amino acid homology is 99.099.099.8% and 97.60.44%, respectively. The isolated EV71 strains belong to C4 subtype.
【作者单位】：吉林大学珠海学院;
【基金】：广东省教育厅“创新强校工程”项目(No.2016KQNCX209)
【分类号】：R725.1

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