硫化氢提高肠道微循环灌注保护新生儿坏死性小肠结肠炎

发布时间：2018-02-14 18:26

本文关键词： 新生儿坏死性小肠结肠炎硫化氢微循环灌注　出处：《中国现代医学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的探讨硫化氢(H_2S)对新生SD大鼠坏死性小肠结肠炎(NEC)的肠道微循环作用。方法出生12 h内的新生SD大鼠10只仅用于检测H_2S合成酶表达,另80只随机分为4组:正常对照组、NEC模型组、NEC+GYY4137组、NEC+AOAA组。各组分别予以腹腔注射生理盐水、LPS+生理盐水、LPS+GYY4137、LPS+AOAA。通过人工喂养-缺氧-冷刺激-脂多糖(LPS)方法复制新生鼠NEC模型。HE染色、病理评分以及动物显微镜拍实体照片显示肠组织损伤程度,并统计分析各组新生鼠生存时间,采用FLPI2激光散斑血流实时成像系统检测各组血流量变化,探讨H_2S对新生鼠NEC的肠道微循环保护作用。结果与模型组比较,当给与NEC处理鼠GYY4137时其肠组织血流量升高(P0.05),病理评分降低(P0.05);而给予AOAA时其病理评分较NEC组增加(P0.05),但肠组织血流量差异并无统计学意义;NEC组较正常组病理评分增高,血流量降低。末端回肠与全肠组织血流量均值差异无统计学意义。结论 H_2S(7.5 mg/kg·d)可通过提高肠道微循环血流量保护NEC,提高NEC新生鼠生存率。
[Abstract]:Objective to investigate the effect of H2S on intestinal microcirculation in neonatal SD rats with necrotizing enterocolitis. Methods Ten neonatal SD rats within 12 hours were used only to detect the expression of H2S synthase. The other 80 rats were randomly divided into 4 groups: normal control group, GYY4137 group, GYY4137 group, intraperitoneal injection of LPS-LPS-GYY4137LPS-AOAA, which were induced by artificial feeding, hypoxia-cold stimulation and lipopolysaccharide (LPS). Neonatal rat NEC model. Pathological scores and animal microscopic images showed the degree of intestinal tissue injury. The survival time of newborn rats in each group was statistically analyzed. The changes of blood flow in each group were detected by FLPI2 laser speckle flow real time imaging system. To investigate the protective effect of H2s on intestinal microcirculation in neonatal rats with NEC. When NEC was given to GYY4137, the intestinal blood flow increased and the pathological score decreased, but the pathological score of AOAA increased than that of NEC group, but there was no significant difference in intestinal blood flow between NEC group and normal group. There was no significant difference in mean blood flow between terminal ileum and total intestinal tissue. Conclusion H2SN 7.5 mg/kg 路d) can improve survival rate of neonatal NEC rats by increasing intestinal microcirculation blood flow.
【作者单位】：武汉大学人民医院儿科;湖北医药学院附属人民医院;湖北医药学院附属人民医院研究所;
【分类号】：R722.1

【相似文献】