MTHFR、ABCB1基因多态性与急性淋巴细胞白血病患儿甲氨蝶呤化疗毒副反应的关系

发布时间：2018-02-20 07:40

本文关键词： 甲氨蝶呤毒副反应基因多态性亚甲基四氢叶酸还原酶三磷酸腺苷结合盒转运体B1　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究亚甲基四氢叶酸还原酶(MTHFR)1298、677位点及三磷酸腺苷结合盒转运体B1(ABCB1)基因多态性与急性淋巴细胞白血病(ALL)患儿大剂量甲氨蝶呤(HDMTX)化疗治疗后发生毒副反应的关系。方法:收集43例2015年2月至2016年12月新疆医科大学第一附属医院的急性淋巴细胞白血病患儿外周血,用聚合酶链反应(PCR)-芯片杂交技术检测患儿MTHFR及ABCB1基因型,同时观察接受HDMTX化疗治疗的ALL患儿发生的毒副反应。结果:MTHFR 677CT+TT基因型患儿发生口腔黏膜损害、骨髓抑制的风险较CC基因型者显著性增高(P0.05);MTHFR 1298AA基因型患儿发生胃肠道反应的风险较AC+CC基因型者显著性增高(P0.05);ABCB1 C3435T基因型为CT+TT的ALL患儿发生骨髓抑制毒副作用的风险高于基因型为CC的患儿(P0.05)。结论:MTHFR、ABCB1基因多态性分布与ALL患儿HDMTX化疗后发生毒副反应有关。
[Abstract]:Objective: to study the relationship between the polymorphism of methylenetetrahydrofolate reductase MTHFRn1298F677 and adenosine triphosphate binding cassette transporter B1 (ABCB1) gene polymorphism in children with acute lymphoblastic leukemia (ALL) and the side effects of high dose methotrexate (HDMTX) chemotherapy in children with acute lymphoblastic leukemia (ALL). Methods: the peripheral blood of 43 children with acute lymphoblastic leukemia from February 2015 to December 2016 in the first affiliated Hospital of Xinjiang Medical University were collected. The genotypes of MTHFR and ABCB1 were detected by polymerase chain reaction (PCR) and PCR microarray hybridization, and the side effects of ALL treated with HDMTX chemotherapy were observed. Results the oral mucosal damage was observed in the children with the ALL genotype of 1: MTHFR677CT. The risk of bone marrow suppression is significantly higher than that of CC genotype. The risk of gastrointestinal reaction in children with MTHFR1298AA genotype is significantly higher than that in patients with AC CC genotype. The side effects of bone marrow suppression in patients with ALL with ABCB1 C3435T genotype of P0.05 or ABCB1 C3435T are significantly higher than those of patients with CT-TT genotype. Conclusion the distribution of ABCB1 gene polymorphism in children with ALL is associated with adverse effects of HDMTX chemotherapy.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R733.71

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