重症手足口病患儿外周血脑微血管内皮细胞计数的变化及意义

发布时间：2018-03-03 15:08

本文选题：重症手足口病　切入点：肠道病毒型　出处：《广东医学》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨普通与重症手足口病患儿外周血脑微血管内皮细胞(BMEC)计数的变化及意义。方法选取8例重症手足口病患儿(重症组),15例普通手足口病患儿(普通组)以及14例非神经系统损伤儿童(对照组)进行分组对照研究,应用免疫磁珠吸附法分离外周血中BMEC,行免疫荧光染色并计数细胞,分析各组患儿外周血BMEC的含量,并分别分析BMEC计数与血液中C反应蛋白(CRP)浓度和白细胞(WBC)含量的关系。结果成功分离出患儿BMEC,其中重症组BMEC、CRP、WBC含量均高于普通组和对照组,差异有统计学意义(F=22.982、5.290、10.631,P0.01)。BMEC含量与WBC含量呈正相关(r=0.921,P0.05)。结论重症手足口病患儿外周血BMEC计数显著高于普通手足口病患儿,外周血BMEC计数有望成为早期诊断重症手足口病的新方法。
[Abstract]:Objective to explore the common and severe hand foot mouth disease in peripheral blood of children with cerebral microvascular endothelial cells (BMEC) changes and significance of counting. Methods 8 cases of severe HFMD patients (severe group), 15 cases of common HFMD patients (normal group) and 14 cases of non nervous system damage in children (control group) were control study group, using immunomagnetic beads adsorption separation of peripheral blood BMEC, immunofluorescence staining and cell counting, analysis of the content of each group in peripheral blood of children with BMEC, were analyzed and BMEC count and blood C reactive protein (CRP) concentration and white blood cell (WBC) content. The results were successfully isolated BMEC, one of the severe group of BMEC, CRP, WBC content were higher than the normal group and the control group, the difference was statistically significant (F=22.982,5.290,10.631, P0.01).BMEC content correlated positively with the content of WBC (r=0.921, P0.05). Conclusion severe HFMD patients peripheral blood BMEC count. The BMEC count in peripheral blood is expected to be a new method for early diagnosis of severe hand foot and mouth disease (HFMD) in children with higher than normal hand foot and mouth disease.

【作者单位】：南方医科大学公共卫生学院广东省热带病研究重点实验室微生物学系;南方医科大学第一临床医学院;
【基金】：广东省科技厅项目(编号:2013B021800032;2016A020215016) 广东大学生科技创新培育专项资金(“攀登计划”专项资金)(编号:pdjh2015b0108) 广东省大学生创新创业训练计划项目(编号:201512121026)
【分类号】：R725.1

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