胶体金法和被动凝集法在肺炎支原体检测中的比较

发布时间：2018-03-04 08:21

  本文选题：肺炎支原体　切入点：胶体金法　出处：《实用医学杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的:比较胶体金法和被动凝集法检测肺炎支原体(MP)感染的符合率,探讨两种方法在快速诊断MP感染中的临床价值。方法:选取MP感染就诊者200例,其中儿童组100例,成年组100例,采用胶体金法和被动凝集法检测两组血清中MP-IgM抗体。结果:在儿童组中被动凝集法MP-IgM抗体阳性检出率略高于胶体金法,但差异无统计学意义(P0.05),而在成年组中被动凝集法阳性检出率则显著高于胶体金法,差异有统计学意义(P0.05);在儿童组中当MP-IgM抗体滴度1∶160,≥1∶320时,胶体金法阳性结果与被动凝集法的符合率分别为95.40%,95.30%;在成年组中当MP-IgM抗体滴度1∶80,1∶160,≥1∶320时,胶体金法阳性结果与被动凝集法的符合率分别为0,61.90%,63.80%。结论:在小儿MP检测中,对于高滴度MP-IgM抗体,胶体金法阳性符合率可达到临床诊断需求。临床医师可根据患者年龄、发病进程选择合适的方法,以实现简便、快速、准确的诊断肺炎支原体感染。
[Abstract]:Objective: to compare the coincidence rate between colloidal gold method and passive agglutination method in the detection of MP infection, and to explore the clinical value of the two methods in the rapid diagnosis of MP infection. Methods: 200 cases of MP infection were selected, including 100 cases in children group. In adult group, MP-IgM antibody in serum was detected by colloidal gold method and passive agglutination method. Results: the positive rate of MP-IgM antibody by passive agglutination method in children group was slightly higher than that by colloidal gold method. However, the positive rate of passive agglutination in adult group was significantly higher than that of colloidal gold method, and the difference was statistically significant when the titer of MP-IgM antibody was 1: 160, 鈮，

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