RSV感染患儿呼吸道上皮细胞中干扰素-λ1的表达水平及其与RSV载量的关系

发布时间：2018-03-04 10:30

本文选题：干扰素-λ　切入点：呼吸道合胞病毒　出处：《中国当代儿科杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的了解呼吸道合胞病毒(RSV)感染患儿呼吸道上皮细胞中干扰素(IFN)-λ1的表达水平及其与RSV载量的关系。方法收集2015年6月至2016年6月期间因呼吸道感染住院患儿的鼻咽拭子标本,采用直接免疫荧光法行7种常见呼吸道病毒(含RSV)抗原检测,筛选出单纯RSV阳性患儿作为RSV感染组(n=120);另收集同期门诊体检健康儿童鼻咽拭子标本,行7种常见呼吸道病毒检查均为阴性的儿童作为健康对照组(n=50)。对两组儿童鼻咽拭子标本行荧光定量PCR检测RSV载量和IFN-λ1 m RNA水平。结果 RSV感染组鼻咽拭子中IFN-λ1 m RNA水平显著高于健康对照组(P0.05),且IFN-λ1 m RNA水平与RSV载量呈正相关(r=0.56,P0.05)。结论 RSV可诱导呼吸道上皮细胞中IFN-λ1的表达,推测IFN-λ1在机体抗RSV感染中可能起重要作用。
[Abstract]:Objective to investigate the expression of IFN- 位 1 in respiratory epithelial cells of children with respiratory syncytial virus (RSV) infection and its relationship with RSV load. Methods the nasopharyngeal swabs of children hospitalized with respiratory tract infection from June 2015 to June 2016 were collected. Seven common respiratory virus (RSVV) antigens were detected by direct immunofluorescence assay, and only RSV positive children were selected as RSV infected group, and nasopharyngeal swabs were collected from healthy children in the same period of outpatient examination. Seven common respiratory virus negative children were used as healthy control group. The RSV load and IFN- 位 1 m RNA level in nasopharynx swab of two groups of children were detected by fluorescence quantitative PCR. Results IFN- 位 1 m in nasopharynx swab of RSV infected group was determined by fluorescence quantitative PCR. The level of RNA was significantly higher than that of control group (P 0.05), and the level of IFN- 位 1 m RNA was positively correlated with the RSV load. Conclusion RSV can induce the expression of IFN- 位 1 in respiratory epithelial cells. It is speculated that IFN- 位 1 may play an important role in RSV infection.
【作者单位】：湖南省人民医院儿童医学中心;
【基金】：湖南省自然科学基金(2017JJ2150) 《中华儿科杂志》百利儿科科研基金(B2016-B-1)
【分类号】：R725.6

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