新疆小儿白血病干细胞免疫表形特点及其相关研究

发布时间：2018-03-04 11:24

本文选题：新疆小儿　切入点：白血病干细胞　出处：《新疆医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：测定维族及汉族患儿白血病干细胞的表达水平（Leukemia Stem Cell LSC），检测免疫表型、染色体核型,探讨其与成人及两个民族间是否存在差异，小儿LSC是否存在新的免疫表型,其与染色体核型之间是否存在关联。为白血病个体化治疗提供理论及实验依据方法：用流式细胞仪测定35例维吾尔族及34例汉族患儿LSC表达水平，其中选择10例汉族患儿及所有维族患儿分选其LSC，体外培养观察其形态学特征并再次通过流式细胞仪检测两个民族LSC新的免疫表型，通过统计学方法进行两个民族CD34+CD38-CD123+细胞表达水平之间是否有差异。结果：两个民族AMLLSC的表达水平在维、汉表达水平之间存在差异（P=0.04）。培养的LSC呈集落样悬浮生长，在体外能存活8个月以上并保持细胞活性，所检测到的表面标记有：急性髓系白血病干细胞（AML LSC）CD34,CD123,CD96,CD44,CD33,CD90.急性淋巴细胞白血病(ALL-LSC)CD34,CD19,CD9,CD90。CD123+，，CD90+在急淋及急非淋LSC中均有不同程度的表达。在维吾尔族染色体核型有：ALL t(12;21),现t(9;22),超二倍体等，AML中t(8;21), t(15:17).资料显示5例M3中4例表达CD96的LSC都出现t(15:17)核型异常结论：AMLCD34+CD38-CD123+细胞表达水平明显低于成人，在维吾尔族及汉族白血病患儿存在差异，ALL LSC除了表达文献相似的免疫表型外，还表达了CD34+CD9+，CD34+CD19+等，这些可能小儿LSC新的免疫表型； CD123+，CD90+可能在急淋及急非淋LSC中均存在;CD96表达可能与t(15:17)异常有关。
[Abstract]:Objective: to determine the expression level of leukemia stem cells in children of Uygur nationality and Han nationality and to detect the immunophenotype and karyotype of leukemia Cell, and to explore whether there are differences between the leukemia stem cells of Uygur nationality and Han nationality, and whether there is a new immunophenotype of LSC in children. To provide theoretical and experimental basis for individualized treatment of leukemia, flow cytometry was used to determine the expression of LSC in 35 children with Uygur nationality and 34 children with Han nationality. Among them, 10 children of Han nationality and all children of Uygur nationality were selected, their morphological characteristics were observed in vitro and the new immunophenotypes of LSC of two nationalities were detected by flow cytometry. Results: the expression level of AMLLSC in the two nationalities was different in Uygur and Han nationality. The cultured LSC was colony like suspension growth. Can survive for more than 8 months in vitro and maintain cell activity. The surface markers detected were as follows: acute myeloid leukemia stem cell (AMLL) LSC-CD34-CD123ONCD96-CD4C4+ CD33-CD90.Acute lymphoblastic leukemia (ALL-LSCT) CD34T CD34T CD19 CD90.CD90.CD90.CD12123 CD90 was expressed in acute lymphoblastic leukemia (AMLL) and acute non-lymphoid leukemia (LSC) to varying degrees. The chromosome karyotype of Uygur nationality has a chromosome karyotype of: all all 1221. The data showed that 4 cases of LSC expressing CD96 in 5 cases of M3 had abnormal karyotype (t 15: 17) conclusion the expression level of CD38-CD123 cell in the CD38-CD123 cell of CD96 was significantly lower than that in the adult. In Uygur and Han nationality children with leukemia, all LSC expressed CD34 CD9 CD34 CD19, in addition to expressing similar immunophenotypes in literature. These new immunophenotypes of LSC may be related to the abnormal expression of CD123 / CD90 in both acute and non-lymphoid LSC.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R733.7

【引证文献】